Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse AraPLEX Experiment Data

Choose an experiment:
Transcriptome changes triggered by Hyaloperonospora parasitica arabidopsis Noco2 in WT and wrky72 mutants at 96 hpt
Kishor Kumar Bhattarai, University of California, Department of Plant Pathology, Riverside, California (bkk33@cornell.edu,thomas.eulgem@ucr.edu)
Experiment design (18 hybridizations)
genotype
•Wildtype •wrky72-1 mutant •wrky72-2 mutant
pathogen infection
•mock •HpaNoco2 treated

This experiment has been imported by PLEXdb from NCBI GEO (GSE18329) Series_summary: Functional characterization of AtWRKY72 using Arabidopsis...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Transcriptome changes triggered by Hyaloperonospora parasitica arabidopsis Noco2 in WT and wrky72 mutants at 96 hpt
Accession No: AT100
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Wildtype   •wrky72-1 mutant   •wrky72-2 mutant
pathogen infection
•mock   •HpaNoco2 treated
Quality Control: biological replicates
Treatment summary:
 genotype  pathogen infection  # replicates
 Wildtype  mock  3
 Wildtype  HpaNoco2 treated  3
 wrky72-1 mutant  mock  3
 wrky72-1 mutant  HpaNoco2 treated  3
 wrky72-2 mutant  mock  3
 wrky72-2 mutant  HpaNoco2 treated  3
Total hybridizations: 18
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE18329)

Series_summary:
Functional characterization of AtWRKY72 using Arabidopsis T-DNA insertion lines showed that this gene is important for basal defense to root-knot nematode (RKN) and Hyaloperonospora parasitica arabidopsis (Hpa), but not several tested R gene-mediated defenses.To profile transcriptional reprogramming associated with AtWRKY72-dependent basal defense we used Affymetrix ATH1 GeneChips representing ~24,000 Arabidopsis genes. Three independent biological replicates were performed with Col-0, wrky72-1 and wrky72-2 plants at 96 hpt with HpaNoco2 or mock treatment. Using a false discovery rate of less than 0.05 we identified for each of these three lines genes that showed significant transcriptional changes in response to HpaNoco2 compared to the mock-treated controls. Identification of downstream targets of WRKY72 in Arabidopsis by this microarray suggests that WRKY72 uses a unique signaling pathway that involves AP2/ERF TFs independent of the ethylene signaling pathway.

Series_overall_design:
We intended to identify Arabidopsis genes that are regulated in WRKY72-dependent manner in response to Hyaloperonospora parasitica arabidopsis (Hpa) at 96 hpt. For this, we used Col-0 and two wrky72 mutant lines and compared the differentially expressed genes with the hypothesis ‘genes that are specifically regulated in Col-0 but not in wrky72 mutants are WRKY72-dependent genes’.
Publication: 'WRKY72-type transcription factors contribute to basal immunity in tomato and Arabidopsis as well as gene-for-gene resistance mediated by the tomato R gene Mi-1.', Bhattarai KK, Atamian HS, Kaloshian I, Eulgem T.
Plant J 2010 Jul;63(2):229-40.
pubmed: 20409007
Created: 2010-11-10 13:27:01
Last Update: 2010-11-10 13:43:59
Released: 2010-11-17
GEO Accession GSE18329
Submitter: PLEXdb Curator
Name: Kishor Kumar Bhattarai
Institution: University of California, Department of Plant Pathology, Riverside, California
Head of Laboratory: Thomas Eulgem
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters