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Expression data from Arabidopsis seed compartments at the linear-cotyledon stage
Ryan Christopher Kirkbride, University of California, Department of Plant Biology, Davis, California (rkirkbride@ucdavis.edu,bobg@ucla.edu)
Experiment design (14 hybridizations)
organism part name
•embryo proper •cellularlized endosperm •chalazal endosperm •chalazal seed coat •general seed coat •whole seeds •micropylar endosperm

This experiment has been imported by PLEXdb from NCBI GEO (GSE12403) Series_summary: We collected linear-cotyledon stage seed compartments fro...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression data from Arabidopsis seed compartments at the linear-cotyledon stage
Accession No: AT103
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
organism part name
•embryo proper   •cellularlized endosperm   •chalazal endosperm   •chalazal seed coat   •general seed coat   •whole seeds   •micropylar endosperm
Quality Control: biological replicates
Treatment summary:
 organism part name  # replicates
 embryo proper  2
 cellularlized endosperm  2
 chalazal endosperm  2
 chalazal seed coat  2
 general seed coat  2
 whole seeds  2
 micropylar endosperm  2
Total hybridizations: 14
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE12403)

Series_summary:
We collected linear-cotyledon stage seed compartments from 5 to 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing linear-coyledon-stage embryos. For the purposes of this study we captured 7 compartments: embyro proper, cellularized endosperm, chalazal endosperm, chalazal seed coat, general seed coat, whole seeds and micropylar endosperm.

Series_overall_design:
linear-cotyledon stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, cellularized endosperm, general seed coat, chalazal seed coat, whole seeds and micropylar endosperm, 2 biological replicates each).

PLEXdb Curator's Note
This sub-series is part of the super-series GSE12404 (Expression Data from Arabidopsis Seed Compartments at 5 Discrete Stages of Development); the sister series are GSE11262 (globular stage), GSE12402 (pre-globular stage), GSE15160 (Heart Stage), GSE15165 (mature green stage), and GSE20039 (bending cotyledon stage).
Publication: none
Created: 2011-02-15 12:38:41
Last Update: 2011-02-15 13:10:13
Released: 2011-02-24
GEO Accession GSE12403
Submitter: PLEXdb Curator
Name: Ryan Christopher Kirkbride
Institution: University of California, Department of Plant Biology, Davis, California
Head of Laboratory: Robert B. Goldberg
email(s):
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