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Expression data from Arabidopsis seed compartments at the heart stage.
Ryan Christopher Kirkbride, University of California, Department of Plant Biology, Davis, California (rkirkbride@ucdavis.edu,bobg@ucla.edu)
Experiment design (16 hybridizations)
organism part name
•embryo proper •micropylar endosperm •peripheral endosperm •chalazal endosperm •chalazal seed coat •general seed coat •whole seeds

This experiment has been imported by PLEXdb from NCBI GEO (GSE15160) Series_summary: We collected heart stage seed compartments from 7 micron ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression data from Arabidopsis seed compartments at the heart stage.
Accession No: AT104
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
organism part name
•embryo proper   •micropylar endosperm   •peripheral endosperm   •chalazal endosperm   •chalazal seed coat   •general seed coat   •whole seeds
Quality Control: biological replicates
Treatment summary:
 organism part name  # replicates
 embryo proper  2
 micropylar endosperm  2
 peripheral endosperm  2
 chalazal endosperm  3
 chalazal seed coat  3
 general seed coat  2
 whole seeds  2
Total hybridizations: 16
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE15160)

Series_summary:
We collected heart stage seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing heart stage embryos. For the purposes of this study we captured 6 compartments: embryo proper, micropylar endosperm, peripheral endosperm, chalazal endosperm, chalazal seed coat and seed coat, as well sets of serial sections encompassing the entire heart stage seed.

Series_overall_design:
Heart stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 16 samples (embryo proper, micropylar endosperm, peripheral endosperm, seed coat and whole seeds, 2 biological replicates each; chalazal endosperm, chalazal seed coat, 3 biological replicates each).

PLEXdb Curator's Note:
This sub-series is part of the super-series GSE12404 (Expression Data from Arabidopsis Seed Compartments at 5 Discrete Stages of Development); the sister series are GSE11262 (globular stage), GSE12402 (pre-globular stage), GSE12403 (linear-cotyledon stage), GSE15165 (mature green stage), and GSE20039 (bending cotyledon stage).
Publication: none
Created: 2011-02-15 13:21:57
Last Update: 2011-02-15 13:47:52
Released: 2011-02-24
GEO Accession GSE15160
Submitter: PLEXdb Curator
Name: Ryan Christopher Kirkbride
Institution: University of California, Department of Plant Biology, Davis, California
Head of Laboratory: Robert B. Goldberg
email(s):
Homepage:

 
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