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Genes regulated by GRF1 and GRF3 in Arabidopsis roots
Tarek Hewezi, Iowa State University (hewezi@iastate.edu)
Experiment design (15 hybridizations)
genotype
•Arabidopsis thaliana ecotypes Col-0 •Arabidopsis thaliana ecotypes WS •Triple mutant grf1/grf2/grf3 •P35S:rGRF1 overexpression •P35S:rGRF3 overexpression

Arabidopsis Affymetrix ATH1 GeneChips were used to compare the mRNA profiles of root tissues of the grf1/grf2/grf3 triple mutant and transgenic pla...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Genes regulated by GRF1 and GRF3 in Arabidopsis roots
Accession No: AT109
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Arabidopsis thaliana ecotypes Col-0   •Arabidopsis thaliana ecotypes WS   •Triple mutant grf1/grf2/grf3   •P35S:rGRF1 overexpression   •P35S:rGRF3 overexpression
Quality Control:
Treatment summary:
 genotype  # replicates
 Arabidopsis thaliana ecotypes Col-0  3
 Arabidopsis thaliana ecotypes WS  3
 Triple mutant grf1/grf2/grf3  3
 P35S:rGRF1 overexpression  3
 P35S:rGRF3 overexpression  3
Total hybridizations: 15
Description: Arabidopsis Affymetrix ATH1 GeneChips were used to compare the mRNA profiles of root tissues of the grf1/grf2/grf3 triple mutant and transgenic plants overexpressing miR396-resistant variants of GRF1 (P35S:rGRF1) or GRF3 (P35S:rGRF3) with those of the corresponding wild-type (Col-0 or WS).
Wild-type (Arabidopsis thaliana ecotypes Col-0 and Ws), the triple mutant grf1/grf2/grf3, and transgenic plants overexpressing rGRF1 or rGRF3 were grown in vertical culture dishes on modified Knop’s medium for 2 weeks and then root tissues were collected for RNA extraction.

Submitted at NCBI GEO via PLEXdb.


Publication: none
Created: 2011-08-12 11:53:03
Last Update: 2011-08-17 12:08:28
Released: 2012-03-13
GEO Accession GSE31593
Submitter: Tarek Hewezi
Name: Tarek Hewezi
Institution: Iowa State University
Head of Laboratory: Thomas Baum
email(s):
Homepage:

 
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