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Transcription profiling of WT and dsr1 Arabidopsis seedlings treated with salicylic acid
Cynthia Gleason, CSIRO, Department of Plant Industry, Wembley, Australia (cynthia.gleason@csiro.au)
Experiment design (12 hybridizations)
genotype
•WT •dsr1
compound
•mock •SA

This experiment has been imported by PLEXdb from NCBI GEO (GSE22942)

Series_summary:
Reactive oxygen species such as hydrogen peroxid...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcription profiling of WT and dsr1 Arabidopsis seedlings treated with salicylic acid
Accession No: AT113
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•WT   •dsr1
compound
•mock   •SA
Quality Control: biological replicates
Treatment summary:
 genotype  compound  # replicates
 WT  mock  3
 WT  SA  3
 dsr1  mock  3
 dsr1  SA  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE22942)

Series_summary:
Reactive oxygen species such as hydrogen peroxide (H2O2) are important in biotic and abiotic stress responses in plants, but their source is often unclear. We have identified an Arabidopsis mutant that shows loss of stress responsive GSTF8 gene expression in response to the plant defence signal salicylic acid (SA) . The mutant showed increased susceptibility to both fungal and bacterial pathogens. The dsr1 mutation was mapped to mitochondrial succinate dehydrogenase (SDH1-1) and dsr1 had reduced SDH activity and a lowered mitochondrial H2O2 production.
To better understand the loss of SA-response in dsr1, we performed ATH1 microarray analysis in WT and dsr1 10 hours after a 1 mM SA treatment, a point at which we observed maximal SA-induced GSTF8 promoter activity.

Series_overall_design:
4 day old WT (a Columbia line containing a -800GSTF8::LUC reporter) and dsr1 seedlings (a mutant Columbia line containing a -800GSTF8::LUC) reporterwere treated with water (mock) or 1 mM SA for 40 min. Ten hours after treatment, samples were collected.
Publication: none
Created: 2012-01-19 10:14:31
Last Update: 2012-01-19 12:13:59
Released: 2012-01-19
GEO Accession GSE22942
Submitter: PLEXdb Curator
Name: Cynthia Gleason
Institution: CSIRO, Department of Plant Industry, Wembley, Australia
Head of Laboratory: Karam B. Singh
email(s):
Homepage:

 
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