Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse AraPLEX Experiment Data

Choose an experiment:
Expression data from Arabidopsis seedlings treated with Pseudomonas aeruginosa (strain PA14)
Suresh Gopalan, Massachusetts General Hosptal & Harvard Medical School, Department of Molecular Biology, Boston, Massachusetts (gopalan@molbio.mgh.harvard.edu,gopalans@resurfx.com)
Experiment design (4 hybridizations)
pathogen strains
•PA14 •PA14-gacA mutant

This experiment has been imported by PLEXdb from NCBI GEO (GSE26084)

Series_summary:
Early stages of host microbe adaptations involve ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Expression data from Arabidopsis seedlings treated with Pseudomonas aeruginosa (strain PA14)
Accession No: AT116
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen strains
•PA14   •PA14-gacA mutant
Quality Control: biological replicates
Treatment summary:
 pathogen strains  # replicates
 PA14  2
 PA14-gacA mutant  2
Total hybridizations: 4
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE26084)

Series_summary:
Early stages of host microbe adaptations involve 'system status changes' (rewiring of pre-existing cellular signaling networks and components) of the host and microbe. We posited that under certain environmental conditions these changes leads to maladaptations and favor emergence of new infectious diseases, and these adaptations will have characteristic signatures representative of the adaptation.
Here using Arabidopsis seedlings in a submerged environment treated with P. aerugionsa, we show one such rewired regulation where the master two-component regulator GacA (previously shown to act upstream of quorum sensing, including the regulator LasR, that in turn controls a subset of virulence factors) is completely dispensable. The gacA mutant behaves similar to wild type P. aeruginosa (strain PA14) by a number of read-outs. Consistent with that, the gene expression data here indicates that the transcriptome pattern of the host is identical when treated with wild type PA14 or PA14-gacA mutant.

Series_overall_design:
Single time point (10 day old Arabidopsis seedlings infected with wild type PA14 or mutant bacteria PA14DgacA, and analyzed 24h after infection) with two independent experimenal replicates per treatment
Publication: none
Created: 2012-01-19 11:05:25
Last Update: 2012-01-25 10:01:05
Released: 2012-01-25
GEO Accession GSE26084
Submitter: PLEXdb Curator
Name: Suresh Gopalan
Institution: Massachusetts General Hosptal & Harvard Medical School, Department of Molecular Biology, Boston, Massachusetts
Head of Laboratory: Frederick M. Ausubel
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters