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Experiment Name:

G3P triggered changes in systemic transcriptome

Accession No:

AT122

Microarray:

ATH1-121501

Visibility:

public

Experiment Type:

Experiment Factor(s):

compound: •mock •G3P

Quality Control:

biological replicates

Treatment summary:

compound	# replicates
mock	3
G3P	3

Total hybridizations: 6

Description:

This experiment has been imported by PLEXdb from NCBI GEO (GSE26973)

Series_summary:
Goal of this study was to determine changes in transcription profile in mock versus G3P treated plants. Arabidopsis (Col-0 ecotype) plants were infiltrated with petiole exudate, with or without G3P, and distal leaves were sampled 24 h post treatments.
Keywords: Glycerol-3-Phosphate, Exudate, Salicylic acid, Arabidopsis, Systemic Acquired Resistance, Defense

Series_overall_design:
3 mock-treated and 3 G3P-treated biological replicates were analyzed (one array each)
Total RNA was isolated from four-week-old plants using TRIZOL method. The experiment was carried out in triplicate and a separate group of plants was used for each set. RNA was processed and hybridized to the Affymetric Arabidopsis ATH1 genome array GeneChip following the manufacturers instructions (http://www.affymetrix.com/Auth/ support/downloads/manuals/expression_analysis_technical_manual.pdf ). All probe sets on the Genechips were assigned hybridization signal above background using Affymetrix Expression Console Software v1.0 (http://www.affymetrix.com/Auth/support/downloads/manuals /expression_console_userguide.pdf ). Data was analyzed using the nonparametric Wilcoxen Rank Sum test. Fold changes are reported as ratios of medians. The P values were calculated individually for each probe set.

Publication:

'Glycerol-3-phosphate is a critical mobile inducer of systemic immunity in plants.', Chanda B, Xia Y, Mandal MK, Yu K, Sekine KT, Gao QM, Selote D, Hu Y, Stromberg A, Navarre D, Kachroo A, Kachroo P.
Nat Genet 2011 May;43(5):421-7.
pubmed: 21441932

Created:

2012-02-14 10:25:24

Last Update:

2012-02-14 11:17:15

Released:

2012-02-14

GEO Accession

GSE26973

Submitter:

PLEXdb Curator

Experimenter:

Name:	Mihir Kumar Mandal
Institution:	University of Kentucky, Department of Plant Pathology, Lexington, Kentucky
Head of Laboratory:	Pradeep Kachroo
email(s):	mkmand2@uky.edu,pk62@uky.edu
Homepage:


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