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Gene expression from Arabidopsis under high light conditions
Francisco Garcia Garcia, CIPF, Department of Bionformatics and Genomics, Valencia, Spain (fgarcia@cipf.es)
Experiment design (9 hybridizations)
stress condition
•control •dark •light

This experiment has been imported by PLEXdb from NCBI GEO (GSE22671)

Series_summary:
We have investigated the genomic response of Ara...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Gene expression from Arabidopsis under high light conditions
Accession No: AT123
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
stress condition
•control   •dark   •light
Quality Control: biological replicates
Treatment summary:
 stress condition  # replicates
 control  3
 dark  3
 light  3
Total hybridizations: 9
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE22671)

Series_summary:
We have investigated the genomic response of Arabidopsis cell suspension culture under high light. Our main goal has been twofold: first, to establish whether chloroplasts in Arabidopsis cell suspension culture are functional and, as such, can act as sensors of adverse external stimuli leading to the activation of genomic defence responses in a manner similar to that described in whole plants exposed to a wide range of environmental stresses and; second, to distinguish which of the ROS that would be probably generated in the chloroplasts is predominant.
Our functional genomic analysis has led us to conclude that singlet oxygen is the major ROS in Arabidopsis cell suspension culture under high light stress and that singlet oxygen production is responsible for a genomic activation associated with the biosynthesis and signalling pathway of several phytohormones that ultimately triggers accelerated cell death.

Series_overall_design:
After the ninth day of growth, a volume of 200 mL of Arabidopsis cell suspension culture with a cell density of approximately 150-200 mg/mL was placed in a glass vessel surrounded by a water bath to maintain constant temperature during the light treatment. Nine microarray experiments were designed and classified as follows: control 1–3 (50 microE/m2/s), 1-h dark 1–3 and high light 1–3 (1800 microE/m2/s); where 1–3 stands for the number of biological replicates. After each treatment, sample RNA was extracted and its quality evaluated. The transcriptomic analysis was performed using Affymetrix GeneChip Arabidopsis genome ATH1 arrays.
Publication: 'Early transcriptional defense responses in Arabidopsis cell suspension culture under high-light conditions.', González-Pérez S, Gutiérrez J, García-García F, Osuna D, Dopazo J, Lorenzo Ó, Revuelta JL, Arellano JB.
Plant Physiol 2011 Jul;156(3):1439-56.
pubmed: 21531897
Created: 2012-02-14 10:51:24
Last Update: 2012-02-14 12:09:54
Released: 2012-02-14
GEO Accession GSE22671
Submitter: PLEXdb Curator
Name: Francisco Garcia Garcia
Institution: CIPF, Department of Bionformatics and Genomics, Valencia, Spain
Head of Laboratory: Juan B. Arellano
email(s):
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