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Effect of gibberellins on metabolism during rosette growth in Arabidopsis
Jos Schippers, MPIMP-golm, Department of Plant Signaling, Potsdam, Germany (schippers@mpimp-golm.mpg.de)
Experiment design (6 hybridizations)
treatment
•control •PBZ •GA

This experiment has been imported by PLEXdb from NCBI GEO (GSE29699)

Series_summary:
To understand the effect of high and low GA leve...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Effect of gibberellins on metabolism during rosette growth in Arabidopsis
Accession No: AT124
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
treatment
•control   •PBZ   •GA
Quality Control: biological replicates
Treatment summary:
 treatment  # replicates
 control  2
 PBZ  2
 GA  2
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE29699)

Series_summary:
To understand the effect of high and low GA levels on plant metabolism and development in Arabidopsis we made use of the GA biosynthesis inhibitor paclobutrazol (PBZ) and exogenously applied GA. The whole genome response at the translation level was assessed by immunopurification of polysomes from PBZ- and GA treated plants expressing FLAG-tagged ribosomal protein L18 (RPL18B). Polysomal associated RNA was isolated and subjected to affymterix ATH1 CHIP analysis. A total of 140 genes were statistically determined to be differentially translated after GA treatment whereas 89 genes where affected PBZ treatment. Our analysis revealed that GA and PBZ have opposing effects on the expression of cell wall and wax layer biosynthesis related genes. In addition, many genes involved in secondary metabolism are upregulated upon PBZ treatment. A set of SAUR-like genes important for mediating auxin responses are downregulated by PBZ, which is of interest to coordinatian of GA levels with growth and development. Interestingly, GA treatment induces the upregulation of transcription factors related to plant defense and senescence, which is in agreement with the early flowering upon GA treatment. Our study provides a first picture of the response of Arabidopsis to altered GA levels at the translation level, and thus will be valuable for understanding gene regulation at the polysome level.

Series_overall_design:
Two replicates for each treatment including GA, PBZ and control samples.
Publication: 'Translatome and metabolome effects triggered by gibberellins during rosette growth in Arabidopsis.', Ribeiro DM, Ara├║jo WL, Fernie AR, Schippers JH, Mueller-Roeber B.
J Exp Bot 2012 Jan 30.
pubmed: 22291129
Created: 2012-02-14 11:47:21
Last Update: 2012-02-16 10:27:44
Released: 2012-02-16
GEO Accession GSE29699
Submitter: PLEXdb Curator
Name: Jos Schippers
Institution: MPIMP-golm, Department of Plant Signaling, Potsdam, Germany
Head of Laboratory: Bernd Muller-Roeber
email(s):
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