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Effect of biotin deficiency on gene expression in Aravbidopsis leaves
Gunter Brader, Austrian Institute of Technology, Health & Environment Department, Seibersdorf, Austria (guenter.brader@ait.ac.at)
Experiment design (9 hybridizations)
genetic line
•wild type •bio4-1
treatment
•water •200µM biotin

This experiment has been imported by PLEXdb from NCBI GEO (GSE26631)

Series_summary:
In addition to its essential metabolic functions...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Effect of biotin deficiency on gene expression in Aravbidopsis leaves
Accession No: AT127
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•wild type   •bio4-1
treatment
•water   •200µM biotin
Quality Control: biological replicates
Treatment summary:
 genetic line  treatment  # replicates
 wild type  water  3
 wild type  200µM biotin  0
 bio4-1  water  3
 bio4-1  200µM biotin  3
Total hybridizations: 9
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE26631)

Series_summary:
In addition to its essential metabolic functions biotin is suggested a critical role in regulating gene expression. The first committed enzyme in biotin biosynthesis in Arabidopsis, 7-keto-8-aminopelargonic acid synthase is encoded by At5g04620 (BIO4). We isolated a novel T-DNA insertion mutant of BIO4 (bio4-1) showing a spontaneous cell death phenotype that could be rescued both by exogenous biotin and genetic complementation. The bio4-1 plants exhibited massive accumulation of hydrogen peroxide.
The T-DNA insertion in the bio4-1 mutant contains a Methionine Sulfoxide Reductase B9 (AT4G21850) under a constitutive 35S CaMV promoter. To dissect the specific effects of biotin defiency on gene expression on a genome wide scale, we compared expression of wt Col-0 leaves with bio4-1 mutants and bio4-1 mutants complemented with D-biotin.

Series_overall_design:
Expression in leaves of Col-0 wt, bio4-1 mutants with a T-DNA insertion in the 5`-UTR of BIO4, and bio4-1 mutants complemented with watering with 200µM D-biotin.
Publication: 'Biotin Deficiency Causes Spontaneous Cell Death and Activation of Defense Signaling.', Li J, Brader G, Helenius E, Kariola T, Palva ET.
Plant J 2011 Nov 29.
pubmed: 22126457
Created: 2012-02-16 11:20:35
Last Update: 2012-02-21 09:54:42
Released: 2012-02-21
GEO Accession GSE26631
Submitter: PLEXdb Curator
Name: Gunter Brader
Institution: Austrian Institute of Technology, Health & Environment Department, Seibersdorf, Austria
Head of Laboratory: E. Tapio Palva
email(s):
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