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Comparison of gene expression profiles between erf5/6 and WT Arabidopsis in response to chitooctaose
Gary Stacey, University of Missouri-Columbia, Department of Plant Sciences, Columbia, Missouri (staceyg@missouri.edu)
Experiment design (12 hybridizations)
genotype
•erf5/6 mutant •WT
treatment
•water •chitooctaose

This experiment has been imported by PLEXdb from NCBI GEO (GSE28227)

Series_summary:
Transcription factors ERF5 and ERF6 may play a r...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Comparison of gene expression profiles between erf5/6 and WT Arabidopsis in response to chitooctaose
Accession No: AT128
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•erf5/6 mutant   •WT
treatment
•water   •chitooctaose
Quality Control: biological replicates
Treatment summary:
 genotype  treatment  # replicates
 erf5/6 mutant  water  3
 erf5/6 mutant  chitooctaose  3
 WT  water  3
 WT  chitooctaose  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE28227)

Series_summary:
Transcription factors ERF5 and ERF6 may play a regulatory role in regulating the expression of chitin-responsive genes. We compared the gene expression profiles between the double mutant erf5/6 and WT plants in response to chitooctaose using the Affymetrix Arabidopsis Whole Genome Arrays.

Series_overall_design:
Both the double mutant erf5/6 and WT plants were grown in liquid MS medium in a growth chamber with constant light. Two-week-old seedlings were treated with chitooctaose at a final concentration of 1 uM for 30 minutes. The controls were similarly treated with an equivalent amount of ddH2O. The treated seedlings were harvested for RNA isolation. Three biological replicates were conducted for the experiment.
Publication: none
Created: 2012-02-21 09:27:47
Last Update: 2012-02-21 10:22:46
Released: 2012-02-21
GEO Accession GSE28227
Submitter: PLEXdb Curator
Name: Gary Stacey
Institution: University of Missouri-Columbia, Department of Plant Sciences, Columbia, Missouri
Head of Laboratory: Gary Stacey
email(s):
Homepage:

 
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