Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse AraPLEX Experiment Data

Choose an experiment:
Transcriptome analysis of vtc1, abi4-102 and wild type Col 0
Pavel Kerchev, University of Leeds, Department of Biological Sciences, Leeds, United Kingdom (pavel.kerchev@scri.ac.uk)
Experiment design (10 hybridizations)
genotype
•Col O •abi4-102 •vtc1

This experiment has been imported by PLEXdb from NCBI GEO (GSE23330)

Series_summary:
The role of abscisic acid (ABA) signalling in th...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Transcriptome analysis of vtc1, abi4-102 and wild type Col 0
Accession No: AT131
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Col O   •abi4-102   •vtc1
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 Col O  3
 abi4-102  3
 vtc1  4
Total hybridizations: 10
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE23330)

Series_summary:
The role of abscisic acid (ABA) signalling in the ascorbic acid (AA)-dependent control of plant growth and defence was determined using the vtc1 and vtc2 mutants, which have impaired ascorbic acid synthesis, and in the abi4 mutant that is impaired in ABA-signalling. ABA levels were increase in the mutants relative to the wild type (Col0). Like vtc1 the vtc2 mutants have a slow growth relative to Col0. However, the wild type phenotype is restored in the abi4vtc2 double mutant. Similarly, the sugar sensing phenotype of in the abi4 is reversed in the abi4vtc2 double mutant. The vtc1 and vtc2 leaf transcriptomes show up to 70 % homology with abi4. Of the transcripts that are altered in the mutants a relative to Col0, only a small number are reversed in the abi4vtc2 double mutants relative to either abi4 or vtc2. We conclude that AA controls growth via an ABA and abi4-dependent signalling pathway. The vtc and abi4 mutants have enhanced glutathione levels and common redox signalling pathways leading to similar gene expression patterns.

Series_overall_design:
Rosettes of 42 days old plants were harvested and used to exctract RNA


Curator's Comment:
At GEO, AT132(GSE23331) is the super-series of the component data-sets, AT130(GSE23329) and AT131(GSE23330).
Publication: 'Kerchev PI, Pellny TK, Vivancos PD, Kiddle G, Hedden P, Driscoll S, Vanacker H, Verrier P, Hancock RD, Foyer CH.', The transcription factor ABI4 Is required for the ascorbic acid-dependent regulation of growth and regulation of jasmonate-dependent defense signaling pathways in Arabidopsis.
Plant Cell 2011 Sep;23(9):3319-34.
pubmed: 21926335
Created: 2012-02-21 10:50:29
Last Update: 2012-02-28 10:56:39
Released: 2012-03-01
GEO Accession GSE23330
Submitter: PLEXdb Curator
Name: Pavel Kerchev
Institution: University of Leeds, Department of Biological Sciences, Leeds, United Kingdom
Head of Laboratory: Christine Foyer
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters