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affy_syringae_malaga_athaliana-Role of different proteases in plant defence
Stephanie Huguet, INRA, Evry, France (huguet@evry.inra.fr)
Experiment design (24 hybridizations)
genotype
•col •ulp1c/ulp1d
pathogen infection
•No treatment •mock •pto-wt •pto-hop •pto-hop-mutant

This experiment has been imported by PLEXdb from NCBI GEO (GSE21920)

Series_summary:
affy_syringae_malaga_athaliana - affy_syringae_m...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: affy_syringae_malaga_athaliana-Role of different proteases in plant defence
Accession No: AT133
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•col   •ulp1c/ulp1d
pathogen infection
•No treatment   •mock   •pto-wt   •pto-hop   •pto-hop-mutant
Quality Control: biological replicates
Treatment summary:
 genotype  pathogen infection  # replicates
 col  No treatment  3
 col  mock  3
 col  pto-wt  3
 col  pto-hop  3
 col  pto-hop-mutant  3
 ulp1c/ulp1d  No treatment  3
 ulp1c/ulp1d  mock  3
 ulp1c/ulp1d  pto-wt  3
 ulp1c/ulp1d  pto-hop  0
 ulp1c/ulp1d  pto-hop-mutant  0
Total hybridizations: 24
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE21920)

Series_summary:
affy_syringae_malaga_athaliana - affy_syringae_malaga_athaliana - - Pseudomonas syringae is a plant pathogenic bacterium that relies on a type III secretion system (T3SS) to cause disease in susceptible hosts and to trigger defence in resistant plants. The T3SS translocates effector proteins directly inside the plant cell. Some P. syringae pathovars translocate the effector HopZ1a, which is recognized in Arabidopsis, triggering the hypersensitive response (HR). This resistance does not depend on the usual defence pathways involved in resistance against other avirulence effectors, so it would be very helpful to know the transcriptomic events that take place in the context of a hypersensitive response triggered by HopZ1a. ULP genes codifies for plant SUMO-proteases. Recent studies by our group revealed that they could be involved in plant defense against microorganisms.-- We infiltrated Arabidopsis thaliana ecotype Columbia wild type with 10mM MgCl2 (as mock inoculation), the virulent pathogen Pseudomonas syringae pv. tomato (Pto), Pto expressing the effector HopZ1a and Pto expressing a HopZ1a derivative carrying a point mutation in a residue essential for the cystein-protease activity. We also grew ulp1c/ulp1d mutant plants to analyze them either without treatment or inoculated with MgCl2 (as mock) and Pto wt.

Keywords: gene knock out,normal vs antisens mutant comparison

Series_overall_design:
24 arrays - ATH1

PLEXdb Curator's Note:
Key:
colmut:col, pto-hop-mutant (3 reps)
colptobis:col, pto-wt (3 reps)
colhop: col, pto-hop (3 reps)
colmock:col, mock (3 reps)
col: col, no treatment (3 reps)
ulp: ulp1c/ulp1d, no treatment (3 reps)
ulpmock: ulp1c/ulp1d, mock (3 reps)
ulppto: ulp1c/ulp1d, pto-wt (3 reps)
ulphop: (0 rep)
ulpmut: (0 rep)
-----------------------
pto: Pseudomonas syringae pv. tomato (Pto)
hop: HopZ1a
Publication: none
Created: 2012-02-28 11:38:09
Last Update: 2012-03-08 10:21:04
Released: 2012-03-08
GEO Accession GSE21920
Submitter: PLEXdb Curator
Name: Stephanie Huguet
Institution: INRA, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
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