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The effects of infection with wild-type or mutant cucumber mosaic virus on the Arabidopsis transcriptome
nasc nasc, NASC, Department of Plant & Crop Sciences, Loughborough, United Kingdom (affy@arabidopsis.org.uk)
Experiment design (9 hybridizations)
pathogen strains
•mock-At •CMV-WT •CMV-A2b

This experiment has been imported by PLEXdb from NCBI GEO (GSE37921)

Series_summary:
The cucumber mosaic virus (CMV) 2b counter-defen...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: The effects of infection with wild-type or mutant cucumber mosaic virus on the Arabidopsis transcriptome
Accession No: AT140
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen strains
•mock-At   •CMV-WT   •CMV-A2b
Quality Control: biological replicates
Treatment summary:
 pathogen strains  # replicates
 mock-At  3
 CMV-WT  3
 CMV-A2b  3
Total hybridizations: 9
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE37921)

Series_summary:
The cucumber mosaic virus (CMV) 2b counter-defense protein disrupts plant antiviral mechanisms mediated by RNA silencing and salicylic acid (SA). Using NASC ATH-1 microarrays to investigate defensive gene expression in 2b-transgenic Arabidopsis thaliana plants we found that, surprisingly, 2b inhibited expression of few SA-regulated genes and in some instances enhanced the effect of SA on certain genes. Strikingly, the 2b protein inhibited changes in the expression of 90% of genes regulated by jasmonic acid (NASCARRAYS-415: Lewsey et al. Molec. Plant-Microbe Interact. vol. 23, in press). We will extend this work to understand the effects of the 2b protein on plant gene expression during an authentic viral infection. By comparing the effects on the transcriptome of infection by wild-type CMV and the 2b gene deletion mutant, CMV∆2b, we will reveal not only the influence of the 2b protein but also the effects of its interactions with other viral gene products (and the process of infection itself), on the host transcriptome.

Series_overall_design:
9 samples were used in this experiment
Publication: none
Created: 2012-07-13 10:42:11
Last Update: 2012-07-13 11:34:47
Released: 2012-07-13
GEO Accession GSE37921
Submitter: PLEXdb Curator
Name: nasc nasc
Institution: NASC, Department of Plant & Crop Sciences, Loughborough, United Kingdom
Head of Laboratory: John Carr
email(s):
Homepage:

 
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