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Analysis of anther development by analysing downstream genes controlled by MS1
Ms Gema Vizcay Barrena , School of Biosciences, University of Nottingham
Experiment design (12 hybridizations)
genotype
•ms1 •Ler-0 wt
tissue type
•Young Flower Buds •Old Flower Buds

This experiment submission is a courtesy of the Nottingham Arabidopsis Stock Centre's microarray database (http://ssbdjc2.nottingham.ac.uk/narrays/...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Analysis of anther development by analysing downstream genes controlled by MS1
Accession No: AT15
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•ms1  •Ler-0 wt
tissue type
•Young Flower Buds  •Old Flower Buds
Quality Control: biological replicates
Treatment summary:
 genotype  tissue type  # replicates
 ms1  Young Flower Buds  3
 ms1  Old Flower Buds  3
 Ler-0 wt  Young Flower Buds  3
 Ler-0 wt  Old Flower Buds  3
Total hybridizations: 12
Description: This experiment submission is a courtesy of the Nottingham Arabidopsis Stock Centre's microarray database (http://ssbdjc2.nottingham.ac.uk/narrays/experimentbrowse.pl).The data was generated from NASC's Affymetrix service and offered for public access at BarleyBase.

If you publish with this data, please reference data from NASC/GARNet:

Craigon DJ., James N., Okyere J., Higgins J., Jotham J., May S.
NASCArrays: A repository for Microarray Data generated by NASC's Transcriptomics Service.
Nucleic Acids Research, (2004). volume 32, Database issue D575-D577.

Following is the original experiment description:

About the Experimenter
Name: Ms Gema Vizcay Barrena
Head of Lab Name: Dr Zoe Wilson
Address: School of Biosciences
University of Nottingham
Sutton Bonington
Loughborough
Leics
Postcode: LE12 5RD
Country: UK
Telephone Number: 0115 9513235
Fax Number: 0115 9516334

Experiment: Analysis of anther development by analysing downstream genes controlled by MS1
Submitted to this database: 23/10/2003

Experiment Description
We have cloned the Arabidopsis MALE STERILITY1 (MS1) gene and shown it to have homology to the PHD-finger class of transcriptional regulators (Wilson et al 2001 Plant J. 28: 27-39). The overall importance of MS1 in pollen development has been clearly demonstrated since when mutated pollen degenerates after microspore release leaving a male sterile but otherwise phenotypically normal plant. We have demonstrated that MS1 is expressed in the tapetum around the stage of microspore release prior to tapetal degeneration. Staging of buds will be defined by bud size. Comparisons of gene expression will be made between the Ler-0 wt and ms1 mutant to identifying changes in expression patterns of genes of critical importance. The identified genes will then be analysed for their expression patterns by RT-PCR and sequence/database homology to identify possible functions. Reverse genetics will be used to obtain the corresponding mutants by hybridising DNA filters of the SLAT and Gene Machine-derived transposon populations with the "identified genes". In situ hybridisations will be conducted using the newly identified geneson wild type and our collection of male sterile mutants to characterise and co-ordinate the patterns of gene expression during anther development.
About this Experiment
Experiment Type: Male sterility
Experimental Parameters:
Parameter Mutant (MS1) / Wildtype
Parameter Flower Bud Size
Quality Control Measures Taken:
no-plants-pooled 20 minimum

All of the data available in this website/database is free, and you are free to do whatever you please with it. If you intend to publish work based on any of this data, please acknowledge us, contact the experimenter above, and either acknowledge them or use them as co-authors in the work.
Publication: none
Created: 2004-06-10 09:33:24
Last Update: 2008-08-05 10:59:10
Submitter: Lishuang Shen
Name: Ms Gema Vizcay Barrena
Institution: School of Biosciences, University of Nottingham
Head of Laboratory: Dr Zoe Wilson
email(s):
Homepage:

 
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