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Carbohydrate- and redox-regulation of gene expression in a TPT mutant
Robin Walters , Dept of Plant Sciences,University of Oxford
Experiment design (12 hybridizations)
dose
•wild type •tpt-1
genotype
•Low light •High light

This experiment submission is a courtesy of the Nottingham Arabidopsis Stock Centre's microarray database (http://ssbdjc2.nottingham.ac.uk/narrays/...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Carbohydrate- and redox-regulation of gene expression in a TPT mutant
Accession No: AT16
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
dose
•wild type  •tpt-1
genotype
•Low light   •High light
Quality Control: biological replicates
Treatment summary:
 dose  genotype  # replicates
 wild type  Low light  3
 wild type  High light  3
 tpt-1  Low light  3
 tpt-1  High light  3
Total hybridizations: 12
Description: This experiment submission is a courtesy of the Nottingham Arabidopsis Stock Centre's microarray database (http://ssbdjc2.nottingham.ac.uk/narrays/experimentbrowse.pl).The data was generated from NASC's Affymetrix service and offered for public access at BarleyBase.

If you publish with this data, please reference data from NASC/GARNet:

Craigon DJ., James N., Okyere J., Higgins J., Jotham J., May S.
NASCArrays: A repository for Microarray Data generated by NASC's Transcriptomics Service.
Nucleic Acids Research, (2004). volume 32, Database issue D575-D577.

Following is the original experiment description from NASCArray:

About the Experimenter
Name: Dr Robin Walters
Head of Lab Name: Dr Nicholas Kruger
Address: Dept of Plant Sciences
University of Oxford
South Parks Road
Oxford

Postcode: OX1 3RB
Country: UK
Telephone Number: 01865 275000
Fax Number: 01865 275074

Experiment: Carbohydrate- and redox-regulation of gene expression in a TPT mutant
Submitted to this database: 27/10/2003
Experiment Description
The triose-phosphate/phosphate translocator (TPT) of the chloroplast inner envelope membrane mediates the counter-exchange of stromal triose phosphates derived from CO2 fixation with cytosolic phosphate, thus providing the cytosol with precursors for sucrose synthesis. We have isolated an Arabidopsis mutant (tpt-1) in which the gene encoding TPT is disrupted by a T-DNA insertion. During growth in low light tpt-1 plants are phenotypically normal, but in high light photosynthesis is inhibited and growth is retarded relative to wildtype. This mutant compensates for the absence of TPT by diverting photosynthate into starch which is hydrolysed and exported from the chloroplast as glucose that is subsequently phosphorylated by hexokinase. In low light the capacity of the pathway of starch synthesis is sufficient to accommodate the normal rate of CO2 fixation, but in high light it is unable to match the potential rate of CO2 fixation. Consequently, in high light-grown plants there are measurable effects on the redoxstate of several components. Thus, the tpt-1 mutation influences the carbohydrate status within the cell, alters the form in which carbon is received by the cytosol, and changes the redox signals that are important in photosynthetic acclimation.Plants will be grown in a 8h light:16h dark regime at both 400 and 100 µmol PAR m-2 s-1. Total RNA will be extracted from pools of individual illuminated leaves from at least eight plants at growth stage 3.70. Leaves will be harvested 2 h into the photoperiod to maximises differences between plant lines in the expression of genes of photosynthesis and carbohydrate metabolism. We anticipate that expression of many genes will be affected by the absence of TPT and by changes in light intensity. However, by comparing differences in transcript levels between wildtype and TPT mutant grown in high light with the differences that occur in plants grown in low light we will discriminate between genes whose expression is affected by changes in the pattern of carbohydrate metabolism and those influenced by redox poise of the thylakoid photochemical components. These comparisons will also highlight genes affected by recently identified regulatory interactions between sugar-sensing and redox-sensing. A genome-wide expression study will establish the extent to which gene expression is altered by the absence of TPT in leaves, and will provide the basis for more detailed analysis of a selected range of transcripts whose levels of expression differ between plant lines.

About this Experiment
Experiment Type: light treatment
Experimental Parameters:
parameter Mutant/Wildtype
Quality Control Measures Taken:
no-plants-pooled 8-12
biological-replicates 3

All of the data available in this website/database is free, and you are free to do whatever you please with it. If you intend to publish work based on any of this data, please acknowledge us, contact the experimenter above, and either acknowledge them or use them as co-authors in the work.
Publication: none
Created: 2004-06-10 09:30:30
Last Update: 2008-08-05 10:59:28
Submitter: Lishuang Shen
Name: Robin Walters
Institution: Dept of Plant Sciences,University of Oxford
Head of Laboratory: Dr Nicholas Kruger
email(s):
Homepage:

 
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