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Penetration resistance in Arabidopsis: Wild-type and ataf1-1 mutant response to Bgh 12 hrs after inoculation, 2*2 factorial design
Michael Krogh Jensen, University of Copenahgen (dbc@life.ku.dk)
Experiment design (12 hybridizations)
genotype
•Wild-type Arabidopsis, ecotype Col-0 •ataf1-1 mutant, ecotype Col-0
treated or untreated
•Un-inoculated •Bgh inoculated

Arabidopsis is a non-host to the biotrophic fungus Blumeria graminis f.sp. hordei (Bgh), effectively hindering Bgh ingress to the epidermal cells b...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Penetration resistance in Arabidopsis: Wild-type and ataf1-1 mutant response to Bgh 12 hrs after inoculation, 2*2 factorial design
Accession No: AT31
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Wild-type Arabidopsis, ecotype Col-0   •ataf1-1 mutant, ecotype Col-0
treated or untreated
•Un-inoculated   •Bgh inoculated
Quality Control: biological replicates
Treatment summary:
 genotype  treated or untreated  # replicates
 Wild-type Arabidopsis, ecotype Col-0  Un-inoculated  3
 Wild-type Arabidopsis, ecotype Col-0  Bgh inoculated  3
 ataf1-1 mutant, ecotype Col-0  Un-inoculated  3
 ataf1-1 mutant, ecotype Col-0  Bgh inoculated  3
Total hybridizations: 12
Description: Arabidopsis is a non-host to the biotrophic fungus Blumeria graminis f.sp. hordei (Bgh), effectively hindering Bgh ingress to the epidermal cells by deposition of callosic papillae formations, i.e. penetration resistance. To better understand the transcriptional changes in Arabidopsis towards Bgh, we compared un-inoculated and inoculated wild-type Arabidopsis samples, with those an ATAF1 mutant allele, compromised in penetration resistance. The experiment included sampling of 8 rosettes for each replicate sample from 6-weeks old plants, 12 hrs after inoculation. A total of 12 biolocigal replicates were sampled with three replicates from each of the four blocks (Wild-type, ataf1-1, Bgh, control).

Submitted at NCBI GEO via PLEXdb.

Publication: 'Transcriptional regulation by a NAC (NAM-ATAF1,2-CUC2) transcription factor attenuates ABA signalling for efficient basal defence towards Blumeria graminis f.sp. hordei in Arabidopsis.', Jensen MK, Hagedorn PH, de Torres-Zabala M, Grant MR, Rung JH, Collinge DB, Lyngkjaer MF
Plant J. 2008 Aug 6. [Epub ahead of print]
pubmed: 18694460
Created: 2008-01-04 08:13:45
Last Update: 2008-01-18 07:56:09
GEO Accession GSE12856
Submitter: Michael Krogh Jensen
Name: Michael Krogh Jensen
Institution: University of Copenahgen
Head of Laboratory: David B. Collinge
email(s):
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