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Arabidopsis thaliana gene expression during floral transition and early flower development
MPI Tübingen, Schmid, Lohmann and Weigel labs, MPI Tübingen
Experiment design (40 hybridizations)
genotype
•Col-0 wildtype with an AG::GUS reporter •Ler with an AG::GUS reporter •co (CS175) •ft (CS184) •lfy-12
organism part
•apical region
developmental stage
•0 day LD •3 day LD •5 day LD •7 day LD

This experiment data was first submitted to TAIR, and is provided by TAIR for public access. Coordinator: Lutz Nover, Detlef Weigel and Thomas Altm...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Arabidopsis thaliana gene expression during floral transition and early flower development
Accession No: AT4
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Col-0 wildtype with an AG::GUS reporter  •Ler with an AG::GUS reporter  •co (CS175)  •ft (CS184)  •lfy-12
organism part
•apical region
developmental stage
•0 day LD  •3 day LD  •5 day LD  •7 day LD
Quality Control: biological replicates
Treatment summary:
 genotype  organism part  developmental stage  # replicates
 Col-0 wildtype with an AG::GUS reporter  apical region  0 day LD  2
 Col-0 wildtype with an AG::GUS reporter  apical region  3 day LD  2
 Col-0 wildtype with an AG::GUS reporter  apical region  5 day LD  2
 Col-0 wildtype with an AG::GUS reporter  apical region  7 day LD  2
 Ler with an AG::GUS reporter  apical region  0 day LD  2
 Ler with an AG::GUS reporter  apical region  3 day LD  2
 Ler with an AG::GUS reporter  apical region  5 day LD  2
 Ler with an AG::GUS reporter  apical region  7 day LD  2
 co (CS175)  apical region  0 day LD  2
 co (CS175)  apical region  3 day LD  2
 co (CS175)  apical region  5 day LD  2
 co (CS175)  apical region  7 day LD  2
 ft (CS184)  apical region  0 day LD  2
 ft (CS184)  apical region  3 day LD  2
 ft (CS184)  apical region  5 day LD  2
 ft (CS184)  apical region  7 day LD  2
 lfy-12  apical region  0 day LD  2
 lfy-12  apical region  3 day LD  2
 lfy-12  apical region  5 day LD  2
 lfy-12  apical region  7 day LD  2
Total hybridizations: 40
Description: This experiment data was first submitted to TAIR, and is provided by TAIR for public access. Coordinator: Lutz Nover, Detlef Weigel and Thomas Altmann.

The details about the AtGenExpress project can be found at: http://www.uni-frankfurt.de/fb15/botanik/mcb/AFGN/atgenex.htm

TAIR's Original Overview About the experiment:

"The MPI Tübingen (Schmid, Lohmann and Weigel labs) have supplied data from 280 Affymetrix arrays, representing 100 experimental treatments."

"This is part of the large collaborative project, AtGenExpress. AtGenExpress was proposed by the German DFG's AFGN (http://www.uni-frankfurt.de/fb15/botanik/mcb/AFGN/AFGNHome.html) project, the US NSF , RIKEN , GARNet, and several smaller groups that will combine resources to use microarray technology to profile gene expression patterns across developmental time and in response to hormones and stress."

"Floral transition and flower development are regulated by numerous environmental and endogenous signals, which are integrated at a relatively small number of floral integrators, such as FLOWERING LOCUS T (FT) and SUPPRESSOR OF CONSTANS OVEREXPRESSION 1 (SOC1). Of the environmental factors, photoperiod is regarded the most important one in promoting floral transition in Arabidopsis thaliana and most labstrains will flower earlier under long day (LD) conditions than under short day (SD) conditions. Arabidopsis is therefore considered a facultative LD plant. To monitor gene expression changes during floral transition and early flower development plants were grown under SD (9 hr light, 15 hr dark) for 30 days. Plants were then shifted to LD (16 hr light, 8 hr dark) conditions to induce flowering. RNA was isolated from micro-dissected apical tissue harvested 0, 3, 5, and 7 days after the shift to LD and double-stranded cDNA was synthesized. Biotinylated cRNA probes were prepared and hybridized to the Affymetrix ATH1 array in duplicate (biological replicates). To study floral transition, we not only analyzed response of wildtype Landsberg erecta (Ler) plants, but also the effect of mutants in the flowering time genes CONSTANS (CO; co-2) and FT (ft-2). Early flower development was analyzed by comparing Col-0 wildtype plants with the meristem identity mutant lfy-12 (Col-0)."

BarleyBase downloaded data from TAIR and make our own processing for public query. Based on the experiment description, we splitted the data set into 2 experiment submissions in BarleyBase: 40 arrays are stored in this experiment (BB17), and another 240 arrays stored in another experiment, BB18.
Publication: none
Created: 2004-06-10 16:49:26
Last Update: 2008-08-05 11:01:56
Submitter: Lishuang Shen
Name: MPI Tübingen
Institution: Schmid, Lohmann and Weigel labs, MPI Tübingen
Head of Laboratory: Coordinator: Prof Lutz Nover
email(s):
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