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The effect of TuMV on translation initiation in Arabidopsis.
Jackson Moeller, Iowa State University (jacksonr@iastate.edu, swhitham@iastate.edu)
Experiment design (12 hybridizations)
pathogen infection
•TuMV inoculated •mock inoculated
RNA fraction
•polysomal RNA •total RNA

Arabidopsis ecotype Col-0 expressing RPL18 fused to His & Flag epitopes (RPL18-HF) under 35S control was used to discern the effects of Turnip mosa...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: The effect of TuMV on translation initiation in Arabidopsis.
Accession No: AT42
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen infection
•TuMV inoculated   •mock inoculated
RNA fraction
•polysomal RNA   •total RNA
Quality Control: biological replicates
Treatment summary:
 pathogen infection  RNA fraction  # replicates
 TuMV inoculated  polysomal RNA  3
 TuMV inoculated  total RNA  3
 mock inoculated  polysomal RNA  3
 mock inoculated  total RNA  3
Total hybridizations: 12
Description: Arabidopsis ecotype Col-0 expressing RPL18 fused to His & Flag epitopes (RPL18-HF) under 35S control was used to discern the effects of Turnip mosaic virus (TuMV) infection on host translation initiation. Plants were grown in LC-1 soil in 12 hour days and infected by TuMV-GFP or mock-inoculated just prior to sending up bolts. Samples were taken from rosette leaves 10 days after inoculation. Only tissue fluorescing GFP was selected from the virus-infected samples. Similar tissue was sampled from mock-infected leaves. FLAG antibody was used to isolate RPL18-HF. The RNA co-immunoprecipitated with RPL18-HF is fully translation-initiated. This translation-initiated RNA, also referred to as polysomal RNA, was isolated and compared to total RNA under both mock and TuMV-infected states to find TuMV-induced changes in host translation initiation.

Submitted at NCBI GEO via PLEXdb
Publication: none
Created: 2009-04-30 11:59:33
Last Update: 2009-05-01 14:38:56
Released: 2011-03-11
GEO Accession GSE20278
Submitter: Jackson R Moeller
Name: Jackson Moeller
Institution: Iowa State University
Head of Laboratory: Steve Whitham
email(s):
Homepage:

 
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