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Tween 20 inducible genes
Gretel,Mara,Hunzicker, Ruhr-Universität, Department:Plant Physiology, Lab:Weiler; Universitätstr. 150, Bochum, NRW 44803, Germany (grechenm@yahoo.com.ar)
Experiment design (6 hybridizations)
compound
•water •Tween20
time
•1 hrs •2.5 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE5513)

Series_summary:Polyethylene glycol sorbitan monoacylates (Tween) are d...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Tween 20 inducible genes
Accession No: AT44
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
compound
•water   •Tween20
time
•1 hrs  •2.5 hrs
Quality Control: biological replicates
Treatment summary:
 compound  time  # replicates
 water  1 hrs  2
 water  2.5 hrs  1
 Tween20  1 hrs  2
 Tween20  2.5 hrs  1
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE5513)

Series_summary:Polyethylene glycol sorbitan monoacylates (Tween) are detergents of widespread use in plant sciences. We show them, notably Tween 20, to cause a rapid and complex change in transcript abundance which bears all characteristics of a PAMP / elicitor-induced defense response, and they do so at concentrations which cause no detectable deleterious effects on plant cellular integrity. The activity does not reside in the intact Tween molecule itself, but is caused by medium-chain fatty acids, notably lauric acid (LA), which are efficiently released from the Tween-backbone by the plant. The Tween / LA-response is independent of the jasmonate signalling system. Medium-chain fatty acids are thus novel elicitors/regulators of plant pathogen defense. The results also have several practical implications: (i) The use of Tweens and, as we show, several other detergents, as solvating/wetting agents on intact plants causes profound physiological changes which may mask actual effects of test compounds; (ii) Tweens by themselves can be regarded (and probably used) as economical, non-toxic, and safe-to-apply elicitors of inducible plant immunity against pathogens.
Keywords: c: plants treated with distilled water as a control. t: plants treated with 1.6 mM f Tween 20

Series_overall_design:
The levels of 118 transcripts were elevated (2-fold) after 1 h, i.e., in both, the IA and IB arrays, but only 12 levels were reduced (by 2-fold). In the 2.5 h array the corresponding numbers were 137 and 11. The transcript levels of 38 genes were found elevated in all three arrays. The validity of the array data was checked for 25 randomly-picked genes out of the 38 genes by RT-PCR.


PLEXdb Curator's Note:
We did not find all the 3 replications for each treatment as has been promised in the experiment design section at GEO.
Publication: 'A novel regulatory system in plants involving medium-chain fatty acids.', Hunzicker GM.
Planta 2009 Dec;231(1):143-53.
pubmed: 19876644
Created: 2010-06-23 14:06:58
Last Update: 2010-06-30 13:26:24
GEO Accession GSE5513
Submitter: PLEXdb Curator
Name: Gretel,Mara,Hunzicker
Institution: Ruhr-Universität, Department:Plant Physiology, Lab:Weiler; Universitätstr. 150, Bochum, NRW 44803, Germany
Head of Laboratory: --
email(s):
Homepage:

 
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