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Targets of AtWRKY27 in Arabidopsis leaves
Mukhtar S, Somssich I, Max.Planck-Institute for Plant Breeding (somssich@mpiz-koeln.mpg.de)
Experiment design (4 hybridizations)
genetic line
•empty-vect-XVE-SALK •empty-vect-XVE-ETL •WK27-XVE-SALK •WK27-XVE-ETL

This experiment has been imported by PLEXdb from NCBI GEO (GSE5537) Series_summary: The Arabidopsis transcription factor WRKY27 was found to...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Targets of AtWRKY27 in Arabidopsis leaves
Accession No: AT46
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•empty-vect-XVE-SALK   •empty-vect-XVE-ETL   •WK27-XVE-SALK   •WK27-XVE-ETL
Quality Control: biological replicates
Treatment summary:
 genetic line  # replicates
 empty-vect-XVE-SALK  1
 empty-vect-XVE-ETL  1
 WK27-XVE-SALK  1
 WK27-XVE-ETL  1
Total hybridizations: 4
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE5537)

Series_summary:

The Arabidopsis transcription factor WRKY27 was found to be involved in plant defense towards Ralstonia solanacearum GMI1000. To identify target genes of WRKY27, we introduced a functional tagged version of WRKY27 into two independent Arabidopsis wrky27 knockout lines (ecotype Columbia) under the control of the estrogen receptor-based chemical-inducible system. 18 days old plants grown on soil (Metro Mix 200) at 22oC under a 10/14 h light/dark cycle were treated for 6h with 10 microM beta-estradiol after which RNA was immediately isolated. The two independent knockout lines transformed with the empty vectors (pMD::XVE-SALK and pMD::XVE-ETL) served as controls and were grown under the same conditions and treated identically as the experimental plants.

Experimenter name = Shahid Mukhtar
Experimenter phone = +49-221-5062-310
Experimenter fax = +49-221-5062-353
Experimenter address = Max.Planck-Institute for Plant Breeding
Experimenter address = Dept. Plant Microbe Interactions
Experimenter address = Carl-von-Linne Weg 10
Experimenter address = Koeln
Experimenter zip/postal_code = 50829
Experimenter country = Germany
Keywords: genetic_modification_design

Series_overall_design:
4 samples were used in this experiment
Publication: 'The Arabidopsis transcription factor WRKY27 influences wilt disease symptom development caused by Ralstonia solanacearum. Plant J. 56, 935-947.', Mukhtar, S.M., Deslandes, L., Auriac, M.-C., Marco, Y., and Somssich, I.E.
Plant J. 56:935-947(2008)
pubmed: 18702671
Created: 2010-07-01 11:22:30
Last Update: 2010-10-13 18:33:20
GEO Accession GSE5537
Submitter: PLEXdb Curator
Name: Mukhtar S, Somssich I
Institution: Max.Planck-Institute for Plant Breeding
Head of Laboratory: Dr. Imre E. Somssich
email(s):
Homepage:

 
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