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Transcriptome changes of Arabidopsis during pathogen and insect attack
Pieterse C, Townsend H, Emmerson Z, Schildknecht B, Utrecht University (--)
Experiment design (14 hybridizations)
pest infestation
•control •avrPstDC3000 •Abrassicicola •Prapae •F-occidentalis •M-persicae
•12 hrs •24 hrs •48 hrs •72 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE5525)
Series_summary: Plant defenses against pathogens and insects are regulat...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Experiment Name: Transcriptome changes of Arabidopsis during pathogen and insect attack
Accession No: AT49
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
pest infestation
•control   •avrPstDC3000   •Abrassicicola   •Prapae   •F-occidentalis   •M-persicae
•12 hrs  •24 hrs  •48 hrs  •72 hrs
Quality Control: biological replicates
Treatment summary:
 pest infestation  time  # replicates
 control  12 hrs  1
 control  24 hrs  1
 control  48 hrs  1
 control  72 hrs  1
 avrPstDC3000  12 hrs  1
 avrPstDC3000  24 hrs  1
 avrPstDC3000  48 hrs  0
 avrPstDC3000  72 hrs  0
 Abrassicicola  12 hrs  0
 Abrassicicola  24 hrs  1
 Abrassicicola  48 hrs  1
 Abrassicicola  72 hrs  0
 Prapae  12 hrs  1
 Prapae  24 hrs  1
 Prapae  48 hrs  0
 Prapae  72 hrs  0
 F-occidentalis  12 hrs  0
 F-occidentalis  24 hrs  1
 F-occidentalis  48 hrs  1
 F-occidentalis  72 hrs  0
 M-persicae  12 hrs  0
 M-persicae  24 hrs  0
 M-persicae  48 hrs  1
 M-persicae  72 hrs  1
Total hybridizations: 14
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE5525)

Plant defenses against pathogens and insects are regulated differentially by cross-communicating signaling pathways in which salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) play key roles. To understand how plants integrate pathogen- and insect-induced signals into specific defense responses, we monitored the dynamics of SA, JA, and ET signaling in Arabidopsis after attack by a set of microbial pathogens and herbivorous insects with different modes of attack. Arabidopsis plants were exposed to a pathogenic leaf bacterium (Pseudomonas syringae pv. tomato), a pathogenic leaf fungus (Alternaria brassicicola), tissue-chewing caterpillars (Pieris rapae), cell-content-feeding thrips (Frankliniella occidentalis), or phloem-feeding aphids (Myzus persicae). Monitoring the signal signature in each plant-attacker combination showed that the kinetics of SA, JA, and ET production varies greatly in both quantity and timing. Analysis of global gene expression profiles demonstrated that the signal signature characteristic of each Arabidopsis-attacker combination is orchestrated into a surprisingly complex set of transcriptional alterations in which, in all cases, stress-related genes are overrepresented. Comparison of the transcript profiles revealed that consistent changes induced by pathogens and insects with very different modes of attack can show considerable overlap. Of all consistent changes induced by A. brassicicola, P. rapae, and F. occidentalis, more than 50% were also induced consistently by P. syringae. Notably, although these four attackers all stimulated JA biosynthesis, the majority of the changes in JA-responsive gene expression were attacker-specific. All together our study shows that SA, JA, and ET play a primary role in the orchestration of the plant's defense response, but other regulatory mechanisms, such as pathway cross-talk or additional attacker-induced signals, eventually shape the highly complex attacker-specific defense response.
In the experimental set up we intended to select for genes that showed a more than 2-fold change in the same direction(up or down)at two time points after pathogen or insect attack.
The results are described in the following paper:
De Vos, M., Van Oosten, V.R., Van Poecke, R.M.P., Van Pelt, J.A., Pozo, M.J., Mueller, M.J., Buchala, A.J., Metraux, J.-P., Van Loon, L.C., Dicke, M. and Pieterse, C.M.J. (2005). Signal signature and transcriptome changes in Arabidopsis upon pathogen and insect attack. Molecular Plant-Microbe Interactions.

Experimenter name = Corne Pieterse
Experimenter phone = +31 30 253 3013
Experimenter fax = +31 30 251 8366
Experimenter department = Section Phytopathology
Experimenter institute = Utrecht University
Experimenter address = Department of Biology
Experimenter address = Utrecht University
Experimenter address = Sorbonnelaan 16
Experimenter address = Utrecht
Experimenter zip/postal_code = 3584CA
Experimenter country = The Netherlands
Keywords: time_series_design; pathogenicity_design

14 samples were used in this experiment
Publication: 'Signal signature anome changes of Arabidopsis during pathogen and insect attack.', De Vos M, Van Oosten VR, Van Poecke RM, Van Pelt JA et al.
Mol. Plant Microbe Interact. 18(9):923-37(2005)
pubmed: 16167763
Created: 2010-07-01 13:40:23
Last Update: 2010-07-01 15:57:31
Released: 2010-07-21
GEO Accession GSE5525
Submitter: PLEXdb Curator
Name: Pieterse C, Townsend H, Emmerson Z, Schildknecht B
Institution: Utrecht University
Head of Laboratory: --

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