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Response to Botrytis cinerea infection
Carine Denoux , Fred Ausubel , Julia Dewdney , Simone Ferrari, AtGenExpress Consortium (--)
Experiment design (12 hybridizations)
pathogen inoculation
•B-Cinerea •Control
time
•18 hrs •48 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE5684) Series_summary: AtGenExpress: A multinational coordinated effort to uncove...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Response to Botrytis cinerea infection
Accession No: AT51
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen inoculation
•B-Cinerea   •Control
time
•18 hrs  •48 hrs
Quality Control: biological replicates
Treatment summary:
 pathogen inoculation  time  # replicates
 B-Cinerea  18 hrs  3
 B-Cinerea  48 hrs  3
 Control  18 hrs  3
 Control  48 hrs  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE5684)

Series_summary:
AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana
The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate.
Responses to B. cinerea infection were assayed in wild-type Col-0 plants at 18 and 48 hours after inoculation of adult leaves. Leaves were inoculated by placing 4 5-ul drops of a 5x10e5 spore solution on each leaf. Control leaves were spotted with droplets of 24g L-1 potato dextrose broth medium.
Experimenter name = Carine Denoux , Fred Ausubel , Julia Dewdney , Simone Ferrari
Experimenter institute = AtGenExpress
Keywords: pathogen series

Series_overall_design:
12 samples were used in this experiment
Publication: none
Created: 2010-07-21 13:49:24
Last Update: 2010-07-21 14:38:47
Released: 2010-07-21
GEO Accession GSE5684
Submitter: PLEXdb Curator
Name: Carine Denoux , Fred Ausubel , Julia Dewdney , Simone Ferrari
Institution: AtGenExpress Consortium
Head of Laboratory:
email(s):
Homepage:

 
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