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Browse AraPLEX Experiment Data

Choose an experiment:
Pseudomonas half leaf injection
Xinnian Dong, -- (--)
Experiment design (32 hybridizations)
pathogen inoculation
•P-syr-avr •P-syr •Mock-MgCl2 •No-treat-control
time
•0 hrs •4 hrs •8 hrs •16 hrs •24 hrs •48 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE5685) Series_summary: AtGenExpress: A multinational coordinated effort to uncove...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Pseudomonas half leaf injection
Accession No: AT52
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen inoculation
•P-syr-avr   •P-syr   •Mock-MgCl2   •No-treat-control
time
•0 hrs  •4 hrs  •8 hrs  •16 hrs  •24 hrs  •48 hrs
Quality Control: biological replicates
Treatment summary:
 pathogen inoculation  time  # replicates
 P-syr-avr  0 hrs  0
 P-syr-avr  4 hrs  2
 P-syr-avr  8 hrs  2
 P-syr-avr  16 hrs  2
 P-syr-avr  24 hrs  2
 P-syr-avr  48 hrs  2
 P-syr  0 hrs  0
 P-syr  4 hrs  2
 P-syr  8 hrs  2
 P-syr  16 hrs  2
 P-syr  24 hrs  2
 P-syr  48 hrs  2
 Mock-MgCl2  0 hrs  0
 Mock-MgCl2  4 hrs  2
 Mock-MgCl2  8 hrs  2
 Mock-MgCl2  16 hrs  2
 Mock-MgCl2  24 hrs  2
 Mock-MgCl2  48 hrs  2
 No-treat-control  0 hrs  2
 No-treat-control  4 hrs  0
 No-treat-control  8 hrs  0
 No-treat-control  16 hrs  0
 No-treat-control  24 hrs  0
 No-treat-control  48 hrs  0
Total hybridizations: 32
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE5685)

Series_summary:
AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana
The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate.
Half of a Columbia plant leaf was injected with Pseudomonas syringae. The other half of the leaf was collected on a time course for analysis.
Experimenter name = Xinnian Dong
Experimenter institute = AtGenExpress
Keywords: pathogen series

Series_overall_design:
32 samples were used in this experiment

PLEXdb Curator's Note:
'0 hour' as a time point has been introduced at PLEXdb to accommodate a factorial structure; it is not a time point in the original design.
Publication: none
Created: 2010-07-21 16:53:21
Last Update: 2010-07-22 10:42:55
Released: 2010-07-28
GEO Accession GSE5685
Submitter: PLEXdb Curator
Name: Xinnian Dong
Institution: --
Head of Laboratory: --
email(s):
Homepage:

 
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