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Agrobacterium tumefaciens-induced tumour development of Arabidopsis thaliana
Rosalia Deeken, -- (--)
Experiment design (4 hybridizations)
pathogen inoculation
•A-tumefaciens-induced tumours •Needle injury control

This experiment has been imported by PLEXdb from NCBI GEO (GSE5725) Series_summary: Agrobacterium tumefaciens, a bacterial species found in tem...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Agrobacterium tumefaciens-induced tumour development of Arabidopsis thaliana
Accession No: AT53
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen inoculation
•A-tumefaciens-induced tumours   •Needle injury control
Quality Control: biological replicates
Treatment summary:
 pathogen inoculation  # replicates
 A-tumefaciens-induced tumours  2
 Needle injury control  2
Total hybridizations: 4
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE5725)

Series_summary:
Agrobacterium tumefaciens, a bacterial species found in temperate soils world wide, is the causative agent of crown gall disease on many plants. A. tumefaciens-induced tumours are feared in orchards and vineyards because of their pathological interference with nutrient and water supply which results in crop decline. Small wounds at the crown of the plant, usually induced by wind-bending, are potential entry sites for the bacterium. The tumorous growth is initiated by the integration and expression of the T-DNA of the bacterial Ti plasmid within the plant nuclear DNA. The T-DNA encodes enzymes catalysing the synthesis of increased concentrations of auxin and cytokinin, and of opines which stimulate cell division and enlargement. The fast growing tumours have been shown to be a strong nutrient sink on their host plants. As a matter of fact, sugar and K+ content were found to be up to 10- and 5-fold, respectively, higher in this tissue and transpiration was about 15 times increased compared to normal tissue. Whereas the morphological structure as well as some physiological and biochemical parameters of the tumour have been analysed in detail, little is known about the underlying gene expression pattern. Proliferation and growth of the tumour induced by Agrobacterium tumefaciens is obviously due to the extraordinary high concentration of phytohormons, minerals and metabolites. Their influence on regulation of gene transcription will provide information on the mechanisms underlying fast tumour growth. In a project funded by the DFG we recently started to investigate the role of solute transporter for tumour development on the model plant Arabidopsis thaliana. By comparing the expression pattern of RNA preparations from Arabidopsis tumour and non-tumour tissue, we will be able to identify genes which facilitate crown gall development. For the expression analysis with an Affymetrix full genome chip we will induce tumours at the base of an injured Arabidopsis inflorescence stalk (var. Wassilewskija, WS-2). RNA will be extraxted from tumour and injured non-tumor inflorescence stalk tissue using the RNeasy Plant Mini Kit (Qiagen), followed by a DNase treatment to eliminate DNA contamination.

This Series record represents a partial dataset. The complete dataset was submitted under GEO accession number GSE13927.

Experimenter name = Rosalia Deeken
Experimenter phone = +49 931 8886121
Experimenter fax = +49 931 8886158
Experimenter institute = Julius-von-Sachs-Institute of Biosciences Molecular Plant Physiology and Electrophysiology
Experimenter address = Julius-von-Sachs-Platz 2
Experimenter address = Wuerzburg
Experimenter zip/postal_code = D-97082
Experimenter country = Germany
Keywords: pathogenicity_design

Series_overall_design:
4 samples were used in this experiment
Publication: none
Created: 2010-07-22 11:05:25
Last Update: 2010-07-22 11:18:05
Released: 2010-07-28
GEO Accession GSE5725
Submitter: PLEXdb Curator
Name: Rosalia Deeken
Institution: --
Head of Laboratory: --
email(s):
Homepage:

 
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