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Expression profiling of A. thaliana wild type Columbia-0 and mutant gh3.5-1D in response to pathogen Pst DC3000(avrRpt2)
Li Qun, Institute of plant physiology & ecology, SIBS, CAS, National Key Laboratory of Plant Molecular Genetics. (--)
Experiment design (12 hybridizations)
genotype
•Col-0 WT •gh3.5-1D Mutant
pathogen inoculation
•Pst DC3000(avrRpt2) •Uninoculated Control

This experiment has been imported by PLEXdb from NCBI GEO (GSE6556) Series_summary: To reveal the underlying molecular mechanism of GH3.5 acti...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression profiling of A. thaliana wild type Columbia-0 and mutant gh3.5-1D in response to pathogen Pst DC3000(avrRpt2)
Accession No: AT64
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Col-0 WT   •gh3.5-1D Mutant
pathogen inoculation
•Pst DC3000(avrRpt2)   •Uninoculated Control
Quality Control: biological replicates
Treatment summary:
 genotype  pathogen inoculation  # replicates
 Col-0 WT  Pst DC3000(avrRpt2)  3
 Col-0 WT  Uninoculated Control  3
 gh3.5-1D Mutant  Pst DC3000(avrRpt2)  3
 gh3.5-1D Mutant  Uninoculated Control  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE6556)

Series_summary:
To reveal the underlying molecular mechanism of GH3.5 action in modulating the SA and auxin pathways, we performed transcriptional profiling of gh3.5-1D plants after infection with or without Pst DC3000(avrRpt2) on a global scale using the Affymetrix Arabidopsis ATH1 GeneChip
Keywords: Pathogen-response

Series_overall_design:
Each 3 leaves of plants of 5-week-old wild type Columbia-0 and mutant gh3.5-1D (heterozygous) were inoculated with Pst DC3000(avrRpt2)at 105 cfu mL-1. Leaves were harvested at 0 h (uninoculated) and 48 hours after inoculation. Three biological repeats were performed on Columbia-0 (wild-type, Col-0) and gh3.5-1D (mutant) after infection with or without Pst DC3000(avrRpt2), respectively.
Publication: 'Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis-Pseudomonas syringae interaction', Zhang Z, Li Q, Li Z, Staswick PE et al
Plant Physiol 2007 Oct;145(2):450-64
pubmed: 17704230
Created: 2010-08-04 11:08:27
Last Update: 2010-08-18 13:44:50
Released: 2010-08-04
GEO Accession GSE6556
Submitter: PLEXdb Curator
Name: Li Qun
Institution: Institute of plant physiology & ecology, SIBS, CAS, National Key Laboratory of Plant Molecular Genetics.
Head of Laboratory: --
email(s):
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