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A LysM Receptor-like Kinase Mediates Chitin Perception and Fungal Resistance in Arabidopsis
Gary Stacey, University of Missouri-Columbia, Plant Sciences Department (--)
Experiment design (12 hybridizations)
strain
•Wildtype •Chitin-Receptor-Mut
treatment
•Chitooctaose •Water

This experiment has been imported by PLEXdb from NCBI GEO (GSE8319) Series_summary: A LysM Receptor-like Kinase Mediates Chitin Perception and ...[complete overview]

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Experiment Name: A LysM Receptor-like Kinase Mediates Chitin Perception and Fungal Resistance in Arabidopsis
Accession No: AT67
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
strain
•Wildtype   •Chitin-Receptor-Mut
treatment
•Chitooctaose   •Water
Quality Control: biological replicates
Treatment summary:
 strain  treatment  # replicates
 Wildtype  Chitooctaose  3
 Wildtype  Water  3
 Chitin-Receptor-Mut  Chitooctaose  3
 Chitin-Receptor-Mut  Water  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE8319)

Series_summary:
A LysM Receptor-like Kinase Mediates Chitin Perception and Fungal Resistance in Arabidopsis

Jinrong Wan,1 Xuecheng Zhang,1 David Neece,2 Katrina M. Ramonell,3 Steve Clough,2,4 Sung-yong Kim,1 Minviluz Stacey,1 and Gary Stacey1*

1Division of Plant Sciences, National Center for Soybean Biotechnology, C.S. Bond Life Sciences Center, University of Missouri-Columbia, Columbia, MO 65211, USA
2Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
3Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35487, USA
4US Department of Agriculture, Soybean/Maize Germplasm, Pathology and Genetics Research, Urbana, IL 61801, USA

*To whom correspondence should be addressed. E-mail: staceyg@missouri.edu

Abstract: Chitin, a polymer of N-acetyl-D-glucosamine, is found in fungal cell walls, but not in plants. Plant cells are capable of perceiving chitin fragments (chitooligosaccharides) to trigger various defense responses. We identified a LysM receptor-like protein (AtLysM RLK1) that is required for the perception of chitooligosaccharides in Arabidopsis. Mutation of this gene blocked the induction of almost all chitooligosaccharide-responsive genes (CRGs) and led to more susceptibility to fungal pathogens, but not to a bacterial pathogen. In addition, exogenously applied chitooligosaccharides enhanced resistance against both fungal and bacterial pathogens in the wild-type plants, but not in the mutant. Together, our data strongly suggest AtLysM RLK1 is the chitin receptor or a key part of the receptor complex and chitin is a PAMP (pathogen-associated molecular pattern) in fungi recognized by the receptor leading to the induction of plant innate immunity against fungal pathogens. Since LysM RLKs were also recently shown to be critical for the perception of the rhizobial lipo-chitin Nod signals, our data suggest that LysM RLKs not just recognize friendly symbiotic rhizobia (via their lipo-chitin Nod signals), but also hostile fungal pathogens (via their cell wall chitin). These data suggest a possible evolutionary relationship between the perception mechanisms of Nod signals and chitin by plants.
Keywords: chitooctaose, chitin receptor mutant

Series_overall_design:
wild type Col-0 and chitin receptor mutants treated with or without chitooctaose
Publication: 'A LysM receptor-like kinase plays a critical role in chitin signaling and fungal resistance in Arabidopsis', Wan J, Zhang XC, Neece D, Ramonell KM et al
Plant Cell 2008 Feb;20(2):471-81
pubmed: 18263776
Created: 2010-08-04 12:51:53
Last Update: 2010-08-18 13:45:29
Released: 2010-08-05
GEO Accession GSE8319
Submitter: PLEXdb Curator
Name: Gary Stacey
Institution: University of Missouri-Columbia, Plant Sciences Department
Head of Laboratory: --
email(s):
Homepage:

 
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