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Comparing gene expression between wild type and trichomeless mutants
Manli Yang Davis, Ohio State University, Plant Cellular and Molecular Biology Department (--)
Experiment design (4 hybridizations)
genotype
•wildtype •gl1 •ttg1 •gl3egl3

This experiment has been imported by PLEXdb from NCBI GEO (GSE9597) Series_summary: Trichomes are found on the leaf surface of many plant spec...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Comparing gene expression between wild type and trichomeless mutants
Accession No: AT69
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•wildtype   •gl1   •ttg1   •gl3egl3
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 wildtype  1
 gl1  1
 ttg1  1
 gl3egl3  1
Total hybridizations: 4
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE9597)

Series_summary:
Trichomes are found on the leaf surface of many plant species. They can be classified into either glandular or non-glandular types. Glandular trichomes are usually multicelluar structures and have long been known to participate in defense against pathogens through the production of specialized chemical products. Non-glandular trichomes, such as those in Arabidopsis thaliana, have been hypothesized to have roles in physical defense against insects, UV protection, detoxification, and heat dispersal. However, it has been difficult to separate a physical role from other possible functions for non-glandular trichomes. We have taken advantage of several mutants in the trichome developmental pathway and gene expression analyses to identify a set of genes expressed predominantly in Arabidopsis trichomes.
Keywords: comparing gene expression between wild type and mutants

Series_overall_design:
We compared the gene expression between the wild type and three trichomeless mutants: gl1, ttg1 and gl3egl1 to see which genes' transcripts are enriched in trichomes.

PLEXdb Curator's Note:
1.
Extra CEL files: While there is no mention of replications in this GEO series (neither in the series description nor in the sample description), the GEO site carries extra CEL files for each of the samples, labeled -1, -2, and -3. Users should note that we have at PLEXdb considered only files labeled -1 for each of the 4 samples. This is because we couldn't unambiguously decide if they were biological or technical replications.
2.
There is ambiguity about one of the genotypes, gl3egl3. This double mutant is mentioned as "gl3egl1" in the Overall design section. PLEXdb has kept the genotype as mentioned in the GSM230348 sample.
Publication: none
Created: 2010-08-18 10:02:28
Last Update: 2010-08-18 13:47:29
Released: 2010-08-19
GEO Accession GSE9597
Submitter: PLEXdb Curator
Name: Manli Yang Davis
Institution: Ohio State University, Plant Cellular and Molecular Biology Department
Head of Laboratory: Erich Grotewold
email(s):
Homepage:

 
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