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Testing Arabidopsis for the presence of arbuscular mycorrhizal signalling pathways
Hsiu-Ling Yap, -- (--)
Experiment design (4 hybridizations)
pathogen inoculation
•G-rosea Spores •Filter Paper Control

This experiment has been imported by PLEXdb from NCBI GEO (GSE10323) Series_summary: Around two-thirds of all plant species form arbuscular my...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Testing Arabidopsis for the presence of arbuscular mycorrhizal signalling pathways
Accession No: AT71
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen inoculation
•G-rosea Spores   •Filter Paper Control
Quality Control: biological replicates
Treatment summary:
 pathogen inoculation  # replicates
 G-rosea Spores  2
 Filter Paper Control  2
Total hybridizations: 4
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE10323)

Series_summary:
Around two-thirds of all plant species form arbuscular mycorrhizasa symbiosis between plant roots and glomalean fungi that leads to the formation of intraradical organs of nutrient exchange and an extraradical network of fungal hyphae effectively extending the plant root system. The mycorrhiza plays a key role in plant nutrition and in enhancing plant resistance against pathogens and improving drought resistance. At present very little is known about the molecular basis of arbuscular mycorrhiza formation. Arabidopsis thaliana (as with all Brassicaceae) does not form arbuscular mycorrhizas (AM). Arabidopsis may either have lost essential gene functions or acquired new ones that prevent a successful symbiotic interaction. However given that mycorrhizal symbiosis developed very early during the evolution of land plants and that many ectomycorrhizal plant species can be colonised by AM fungi it is likely that important components of AM signalling pathways are conserved in all plants including Arabidopsis. Possibly the lack of AM development is a multigenic trait and this would make it difficult to isolate mutants that (re-)gain the ability to interact with AM fungi. What can be done however is firstly to test which parts of one or several putative AM signalling pathways are still functional and which ones are not. Secondly we can test whether negatively acting pathways such as those involved in defence against pathogenic microorganisms are induced upon inoculation with AM fungi. Together this work is likely to give important information of why Arabidopsis (and Brassicaceae) are behaving as non-hosts for AM fungi. In other words Arabidopsis will be an ideal system to study mechanisms of non-host "resistance" to AM colonisation. Elucidating these mechanisms will obviously make a great contribution to understanding the basis of the mycorrhizal interaction. Moreover using Arabidopsis as a tool it will be possible at the end to integrate the information obtained for AM signalling with that obtained for other developmental and environmentally triggered signalling pathways such as plant hormone signalling or plant defence responses. To produce an inventory of which Arabidopsis genes respond at all to inoculation with AM fungi a genome-wide screen for AM-controlled genes is proposed. RNA will be prepared from Arabidopsis roots treated with AM fungus and mock-inoculated control plants. Arabidopsis (Col-0) will be grown in pot culture (1:1 sand/Terra-Green) at low concentrations of phosphate. Three week-old plants will be inoculated with surface-sterilised spores of Gigaspora rosea. RNA will be isolated 3 days post inoculation.

Experimenter name: Hsiu-Ling Yap
Experimenter phone: 01904 434 302/304
Experimenter fax: 01904 434 312
Experimenter institute: University of York
Experimenter address: Department of Biology
University of York
P.O.Box 373
York!Series_summary = Experimenter zip/postal_code: YO10 5YW
Experimenter country: UK
Keywords: compound_treatment_design

Series_overall_design:
4 samples were used in this experiment
Publication: none
Created: 2010-08-18 11:53:35
Last Update: 2010-08-18 12:04:14
Released: 2010-08-20
GEO Accession GSE10323
Submitter: PLEXdb Curator
Name: Hsiu-Ling Yap
Institution: --
Head of Laboratory: --
email(s):
Homepage:

 
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