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Golovinomyces orontii time course with Col-0 and eds16-1
Mary C Wildermuth, University of California Berkeley, Plant and Microbial Biology Department (mwildermuth@berkeley.edu)
Experiment design (56 hybridizations)
genotype
•Col-0 •eds16-1
pathogen inoculation
•G-orontii •Uninoculated
time
•0 hai •6 hai •1 dpi •3 dpi •5 dpi •7 dpi

This experiment has been imported by PLEXdb from NCBI GEO (GSE13739) Series_summary: Salicylic acid (SA) is a critical molecule mediating plan...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Golovinomyces orontii time course with Col-0 and eds16-1
Accession No: AT74
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Col-0   •eds16-1
pathogen inoculation
•G-orontii   •Uninoculated
time
•0 hai  •6 hai  •1 dpi  •3 dpi  •5 dpi  •7 dpi
Quality Control: biological replicates
Treatment summary:
 genotype  pathogen inoculation  time  # replicates
 Col-0  G-orontii  0 hai  0
 Col-0  G-orontii  6 hai  4
 Col-0  G-orontii  1 dpi  4
 Col-0  G-orontii  3 dpi  4
 Col-0  G-orontii  5 dpi  4
 Col-0  G-orontii  7 dpi  4
 Col-0  Uninoculated  0 hai  4
 Col-0  Uninoculated  6 hai  0
 Col-0  Uninoculated  1 dpi  0
 Col-0  Uninoculated  3 dpi  0
 Col-0  Uninoculated  5 dpi  0
 Col-0  Uninoculated  7 dpi  4
 eds16-1  G-orontii  0 hai  0
 eds16-1  G-orontii  6 hai  4
 eds16-1  G-orontii  1 dpi  4
 eds16-1  G-orontii  3 dpi  4
 eds16-1  G-orontii  5 dpi  4
 eds16-1  G-orontii  7 dpi  4
 eds16-1  Uninoculated  0 hai  4
 eds16-1  Uninoculated  6 hai  0
 eds16-1  Uninoculated  1 dpi  0
 eds16-1  Uninoculated  3 dpi  0
 eds16-1  Uninoculated  5 dpi  0
 eds16-1  Uninoculated  7 dpi  4
Total hybridizations: 56
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE13739)

Series_summary:
Salicylic acid (SA) is a critical molecule mediating plant innate immunity with an important role limiting the growth and reproduction of the virulent powdery mildew (PM) Golovinomyces orontii on Arabidopsis thaliana. To investigate this later phase of the PM interaction, and the role played by SA, we performed replicated global expression profiling for wild type and SA biosynthetic mutant ics1 Arabidopsis from 0 to 7 days post infection. We found that ICS1-impacted genes comprise 3.8% of profiled genes with known molecular markers of Arabidopsis defense ranked very highly by the multivariate empirical Bayes statistic (T2 statistic ((Tai and Speed, 2006)). Functional analyses of T2-selected genes identified statistically significant PM-impacted processes including photosynthesis, cell wall modification, and alkaloid metabolism that are ICS1-independent. ICS1-impacted processes include redox, vacuolar transport/secretion, and signaling. Our data also supports a role for ICS1 (SA) in iron and calcium homeostasis and identifies components of SA crosstalk with other phytohormones. Through our analysis, 39 novel PM–impacted transcriptional regulators were identified. Insertion mutants in one of these regulators, PUX2, results in significantly reduced reproduction of the powdery mildew in a cell death independent manner. Though little is known about PUX2, PUX1 acts as a negative regulator of Arabidopsis CDC48 (Rancour et al., 2004; Park et al., 2007), an essential AAA-ATPase chaperone that mediates diverse cellular activities including homotypic fusion of ER and Golgi membranes, ER-associated protein degradation, cell cycle progression, and apoptosis. Future work will elucidate the functional role of the novel regulator PUX2 in PM resistance.

Keywords: time course, pathogen response

Series_overall_design:
Arabidopsis whole leaves were harvested at 6h, 1 day, 3 days, 5 days and 7 days after Golovinomyces orontii infection for RNA extraction and hybridization to Affymetrix Arabidopsis ATH1 microarrays. Gene expression profiles were obtained for wild type Columbia-0 and enhanced disease susceptibility mutant eds16-1, a null isochorismate synthase 1 (At1g74710) mutant. In parallel, uninfected samples were collected at 0 hr and 7days from wild type and mutant plants. The experiment includes 4 biological replicates.
Publication: 'Temporal global expression data reveal known and novel salicylate-impacted processes and regulators mediating powdery mildew growth and reproduction on Arabidopsis', Chandran D, Tai YC, Hather G, Dewdney J et al
Plant Physiol 2009 Mar;149(3):1435-51
pubmed: 19176722
Created: 2010-08-18 14:45:10
Last Update: 2010-08-18 15:49:27
Released: 2010-08-24
GEO Accession GSE13739
Submitter: PLEXdb Curator
Name: Mary C Wildermuth
Institution: University of California Berkeley, Plant and Microbial Biology Department
Head of Laboratory: --
email(s):
Homepage:

 
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