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Transcriptome changes triggered by the synthetic defense elicitors DCA and INA in Arabidopsis thaliana
Thomas Eulgem, University of California, Department of Botany and Plant Sciences (thomas.eulgem@ucr.edu)
Experiment design (24 hybridizations)
strain
•Col-0 •npr1-3
treatment
•DCA •INA •Mock
time
•2 days •6 days

This experiment has been imported by PLEXdb from NCBI GEO (GSE13833) Series_summary: DCA (3,5-Dichloroanthranilic acid) is a newly identified ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcriptome changes triggered by the synthetic defense elicitors DCA and INA in Arabidopsis thaliana
Accession No: AT75
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
strain
•Col-0   •npr1-3
treatment
•DCA   •INA   •Mock
time
•2 days  •6 days
Quality Control: biological replicates
Treatment summary:
 strain  treatment  time  # replicates
 Col-0  DCA  2 days  3
 Col-0  DCA  6 days  3
 Col-0  INA  2 days  3
 Col-0  INA  6 days  3
 Col-0  Mock  2 days  3
 Col-0  Mock  6 days  3
 npr1-3  DCA  2 days  3
 npr1-3  DCA  6 days  0
 npr1-3  INA  2 days  0
 npr1-3  INA  6 days  0
 npr1-3  Mock  2 days  3
 npr1-3  Mock  6 days  0
Total hybridizations: 24
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE13833)

Series_summary:
DCA (3,5-Dichloroanthranilic acid) is a newly identified synthetic defense elicitor. To perform a comparative analysis of defense responses triggered by DCA and the structurally related defense inducer INA (2,6-Dichloroisonicotinic acid) Affymetrix chip experiments were performed with Arabidopsis thaliana seedlings treated with one of these two compounds.

Series_overall_design:
Arabidopsis thaliana plants (accession Col-0) were grown on soil in a semi-sterile growth chamber under fluorescent lights (14 hours light, 10 hours dark, 21 centi grades, 100 Einstein/m2/s). Wild type Col-0 plants and the npr1-3 mutant were used in this study. Aerial tissues of 2 week old soil grown Arabidopsis seedlings were sprayed with 100uM 3,5-Dichloroanthranilic acid (DCA), or 100uM 2,6-Dichloroisonicotinic acid (INA) or mock solution and harvested 48h or 6 days after the treatment. Final DMSO concentrations never exceeded 0.002%. Mock treatments were application of 0.002% DMSO in water. For harvesting the aerial plant parts were shock-frozen in liquid nitrogen. Total RNA was isolated from seedlings using TRIZOL (Invitrogen) follwing the manufacturer’s instructions. RNA was processed and hybridized to Affymetrix Arabidopsis ATH1 genome array GeneChip following manufacturer’s instructions (Affymetrix) by the University of California, Riverside Core Instrument Facility. Three independent biological replicates were performed for each treatment.
Publication: 'The synthetic elicitor 3,5-dichloroanthranilic acid induces NPR1-dependent and NPR1-independent mechanisms of disease resistance in Arabidopsis', Knoth C, Salus MS, Girke T, Eulgem T
Plant Physiol 2009 May;150(1):333-47
pubmed: 19304930
Created: 2010-08-19 16:39:10
Last Update: 2010-08-23 13:07:21
Released: 2010-08-24
GEO Accession GSE13833
Submitter: PLEXdb Curator
Name: Thomas Eulgem
Institution: University of California, Department of Botany and Plant Sciences
Head of Laboratory: Thomas Eulgem
email(s):
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