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Transcriptome of mature Arabidopsis thaliana crown galls
Rosalia Deeken, University of Wuerzburg, Julius-von-Sachs-Institute, Botany I; Plant Molecular Physiology & Biophysics Department (deeken@botanik.uni-wuerzburg.de)
Experiment design (8 hybridizations)
pathogen inoculation
•mock •Agr-tum-C58-inoculated

This experiment has been imported by PLEXdb from NCBI GEO (GSE13927) Series_summary: This study describes physiological changes, morphological...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcriptome of mature Arabidopsis thaliana crown galls
Accession No: AT76
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen inoculation
•mock   •Agr-tum-C58-inoculated
Quality Control: biological replicates
Treatment summary:
 pathogen inoculation  # replicates
 mock  4
 Agr-tum-C58-inoculated  4
Total hybridizations: 8
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE13927)

Series_summary:
This study describes physiological changes, morphological adaptations and the regulation of pathogen defense responses in Arabidopsis crown galls. Crown gall development was induced on intact plants under most natural conditions with Agrobacterium tumefaciens. Differential gene expression and the metabolite pattern was determined by comparing crown galls with mock-inoculated inflorescence stalk segments of the same age.

Series_overall_design:
The bases of Arabidopsis thaliana (WS-2) inflorescence stalks were wounded and immediately inoculated with Agrobacterium tumefaciens, strain C58, or mock-inoculated. Plants were cultivated for another 35 days under short day conditions (8 h illumination, 16 h darkness). Gene expression values of four independent experiments of treated material (C58 35dpi 1 to 4) were compared with four non-treated samples of the same age (reference 35dpi 1 to 4). Differential gene expression was analyzed by applying the LIMMA package (Linear Models for Microarray Data; Smyth, G.K. (2004) Applic. Genet. Mol. Biol. 3, Article 3; http://www.bepress.com/sagmb/vol3/iss1/art3/).
PLEXdb Curator's Note:
This experiment is a subseries of GSE14106: Transcriptome data from inflorescence stalks of intact A. thaliana plants after infection with A. tumefaciens.
Publication: 'A central role of abscisic acid in drought stress protection of Agrobacterium-induced tumors on Arabidopsis', Efetova M, Zeier J, Riederer M, Lee CW, Stingl N, Mueller M, Hartung W, Hedrich R, Deeken R.
Plant Physiol 2007 Nov;145(3):853-62.
pubmed: 17827272
'An integrated view of gene expression and solute profiles of Arabidopsis tumors: a genome-wide approach', Deeken R, Engelmann JC, Efetova M, Czirjak T, Müller T, Kaiser WM, Tietz O, Krischke M, Mueller MJ, Palme K, Dandekar T, Hedrich R.
Plant Cell 2006 Dec;18(12):3617-34
pubmed: 17172353
Created: 2010-08-23 13:19:55
Last Update: 2010-08-23 14:22:22
Released: 2010-08-26
GEO Accession GSE13927
Submitter: PLEXdb Curator
Name: Rosalia Deeken
Institution: University of Wuerzburg, Julius-von-Sachs-Institute, Botany I; Plant Molecular Physiology & Biophysics Department
Head of Laboratory: Prof. Hedrich
email(s):
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