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Arabidopsis thaliana three hours after infection with Agrobacterium tumefaciens
Rosalia Deeken, University of Wuerzburg, Julius-von-Sachs-Institute, Botany I; Plant Molecular Physiology & Biophysics Department (deeken@botanik.uni-wuerzburg.de)
Experiment design (15 hybridizations)
treatment
•mock •Agr-tum-C58-inoculated •Agr-tum-GV3101-inoculated

This experiment has been imported by PLEXdb from NCBI GEO (GSE13929) Series_summary: This study focuses on responses of the host plant to infe...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Arabidopsis thaliana three hours after infection with Agrobacterium tumefaciens
Accession No: AT77
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
treatment
•mock   •Agr-tum-C58-inoculated   •Agr-tum-GV3101-inoculated
Quality Control: biological replicates
Treatment summary:
 treatment  # replicates
 mock  6
 Agr-tum-C58-inoculated  6
 Agr-tum-GV3101-inoculated  3
Total hybridizations: 15
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE13929)

Series_summary:
This study focuses on responses of the host plant to infection with Agrobacterium tumefaciens. Genome wide changes in gene expression were integrated with the alterations in metabolite levels three hours after inoculation of agrobacteria. Plants were infected with the virulent strain C58, harboring a T-DNA, or with strain GV3101, containing a disarmed Ti-plasmid. This allows discrimination between signals which derive from the bacterial pathogen and the T-DNA encoded genes.

Series_overall_design:
The bases of Arabidopsis thaliana (WS-2) inflorescence stalks were wounded and immediately infected with Agrobacterium tumefaciens or mock-infected for three hours. Stalks of intact plants were inoculated with the oncogenic strain C58 (C58 3 hpi 1 to 6) or the non-virulent strain GV3101 (GV3101 3 hpi 1 to 3) to provide conditions close to nature. The gene expression data of three independent experiments of infected material were compared with six non-infected samples (reference 3 hpi 1 to 6). Differential gene expression was determined by applying the LIMMA package (Linear Models for Microarray Data; Smyth, G.K. (2004) Applic. Genet. Mol. Biol. 3, Article 3; http://www.bepress.com/sagmb/vol3/iss1/art3/).
PLEXdb Curator's Note:

This experiment is a subseries of GSE14106: Transcriptome data from inflorescence stalks of intact A. thaliana plants after infection with A. tumefaciens.

Publication: 'Agrobacterium tumefaciens promotes tumor induction by modulating pathogen defense in Arabidopsis thaliana', Lee CW, Efetova M, Engelmann JC, Kramell R, Wasternack C, Ludwig-Müller J, Hedrich R, Deeken R.
Plant Cell. 2009 Sep;21(9):2948-62. Epub 2009 Sep 30.
pubmed: 19794116
'An integrated view of gene expression and solute profiles of Arabidopsis tumors: a genome-wide approach', Deeken R, Engelmann JC, Efetova M, Czirjak T, Müller T, Kaiser WM, Tietz O, Krischke M, Mueller MJ, Palme K, Dandekar T, Hedrich R.
Plant Cell 2006 Dec;18(12):3617-34
pubmed: 17172353
Created: 2010-08-23 14:35:03
Last Update: 2010-08-23 15:02:50
Released: 2010-08-26
GEO Accession GSE13929
Submitter: PLEXdb Curator
Name: Rosalia Deeken
Institution: University of Wuerzburg, Julius-von-Sachs-Institute, Botany I; Plant Molecular Physiology & Biophysics Department
Head of Laboratory: Prof. Hedrich
email(s):
Homepage:

 
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