Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse AraPLEX Experiment Data

Choose an experiment:
Expression profiling of the Arabidopsis Mediator complex mutant pft1/med25 and wildtype infected with Fusarium oxysporum
Brendan Kidd, CSIRO, Plant Industry Department (brendan.kidd@csiro.au)
Experiment design (16 hybridizations)
pathogen inoculation
•Mock •F-oxysporum
genotype
•Col-0-WT •pft1-mut

This experiment has been imported by PLEXdb from NCBI GEO (GSE15236) Series_summary: The Mediator complex is an evolutionary conserved multipr...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Expression profiling of the Arabidopsis Mediator complex mutant pft1/med25 and wildtype infected with Fusarium oxysporum
Accession No: AT79
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen inoculation
•Mock   •F-oxysporum
genotype
•Col-0-WT   •pft1-mut
Quality Control: biological replicates
Treatment summary:
 pathogen inoculation  genotype  # replicates
 Mock  Col-0-WT  4
 Mock  pft1-mut  4
 F-oxysporum  Col-0-WT  4
 F-oxysporum  pft1-mut  4
Total hybridizations: 16
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE15236)

Series_summary:
The Mediator complex is an evolutionary conserved multiprotein complex that plays an essential role in initiating and regulating transcription. Its function is to act as a universal adaptor between RNA Polymerase II and DNA-bound transcription factors to translate regulatory information from activators and repressors to the transcriptional machinery. We have found that the PFT1 gene (which encodes the MED25 subunit of the Mediator complex) is required for the uncompromised expression of both salicylic acid- and jasmonate-dependent defense genes as well as resistance to the leaf-infecting fungal pathogens, Alternaria brassicicola and Botrytis cinerea in Arabidopsis. Surprisingly, we found that the pft1/med25 mutant showed increased resistance to the root infecting pathogen Fusarium oxysporum and that this resistance was independent of classical defense genes. In addition, the over-expression of PFT1 led to increased susceptibility to F. oxysporum. Therefore, to explore this phenomenon further, we wished to use whole genome transcript profiling to identify which genes may be playing a role in pft1/med25-mediated resistance to F. oxysporum.

Series_overall_design:
We grew both WT and pft1/med25 plants for four weeks in soil. After four weeks we treated the plants by root-dipping in either water or a F. oxysporum spore suspension before replanting them back into soil. There were four independent replicates of each treatment and each replicate contained 20 plants each. The treatments were WT (mock): pft1 (mock): WT (infected): pft1 (infected). Each replicate (16 in total) was harvested after 48 hours and the resulting RNA was used for hybridization to an Affymetrix ATH1 chip.
Publication: 'The mediator complex subunit PFT1 is a key regulator of jasmonate-dependent defense in Arabidopsis', Kidd BN, Edgar CI, Kumar KK, Aitken EA et al
Plant Cell 2009 Aug;21(8):2237-52
pubmed: 19671879
Created: 2010-08-24 11:43:38
Last Update: 2010-08-24 12:03:24
Released: 2010-08-31
GEO Accession GSE15236
Submitter: PLEXdb Curator
Name: Brendan Kidd
Institution: CSIRO, Plant Industry Department
Head of Laboratory: --
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters