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Expression analysis of Arabidopsis suspension cells during sucrose starvation.
Diane Bassham, Iowa (bassham@iastate.edu)
Experiment design (9 hybridizations)
compound
•Plus Sucrose •Minus Sucrose •Minus Suc/Plus PEG
time
•0 hour •24 hour •48 hour

Arabidopsis suspension cells were grown for 48 hours in medium that contained or lacked sucrose. Total RNA samples were used to perform Affymetrix...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression analysis of Arabidopsis suspension cells during sucrose starvation.
Accession No: AT8
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
compound
•Plus Sucrose  •Minus Sucrose  •Minus Suc/Plus PEG
time
•0 hour  •24 hour  •48 hour
Quality Control: biological replicates
Treatment summary:
 compound  time  # replicates
 Plus Sucrose  0 hour  2
 Plus Sucrose  48 hour  2
 Minus Sucrose  24 hour  2
 Minus Sucrose  48 hour  2
 Minus Suc/Plus PEG  48 hour  1
Total hybridizations: 9
Description: Arabidopsis suspension cells were grown for 48 hours in medium that contained or lacked sucrose. Total RNA samples were used to perform Affymetrix GeneChip Analysis with ATH1 chips. Sample designations are listed below:

BASSHAM 1 = 0 hour plus sucrose
BASSHAM 2 = 24 hour no sucrose
BASSHAM 3 = 48 hour no sucrose
BASSHAM 4 = 48 hour plus sucrose
BASSHAM 5 = 48 hour no sucrose plus PEG (osmotic control)

Publication: 'Transcriptome profiling of the response of Arabidopsis thaliana suspension culture cells to sucrose starvation', Contento AL, Kim SJ and Bassham DC
Plant Physiology, 135(4), 2330-47
pubmed: 15310832
Created: 2004-10-25 11:17:18
Last Update: 2008-08-05 11:02:43
Submitter: Diane C Bassham
Name: Diane Bassham
Institution: Iowa
Head of Laboratory:
email(s):
Homepage:

 
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