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Identification of early flg22 responsive genes in Arabidopsis mesophyll cells
Jen Sheen, Massachusetts General Hospital, Molecular Biology Department (sheen@molbio.mgh.harvard.edu)
Experiment design (6 hybridizations)
time
•Control 0 mins •30 mins •60 mins

This experiment has been imported by PLEXdb from NCBI GEO (GSE16472) Series_summary: The goal of this experiment was to identify the early res...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Identification of early flg22 responsive genes in Arabidopsis mesophyll cells
Accession No: AT82
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
time
•Control 0 mins  •30 mins  •60 mins
Quality Control: biological replicates
Treatment summary:
 time  # replicates
 Control 0 mins  2
 30 mins  2
 60 mins  2
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE16472)

Series_summary:
The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis mesophyll cells that are involved in the initial responses important for plant innate immunity.
We used the microarrays to detail the global program of gene expression underlying intial cellular and molecular responses to microbial associated molecualr patterns (MAMPs).

Series_overall_design:
Arabidopsis mesophyll protoplasts were treated with 100 nM flg22 for 30 min and 60 min. Transcript profiles were analyzed and compared between control and treated cells to identify early flg22 responsive genes.

PLEXdb Curator's Note:

The three experiments, AT81, AT82, AT85 are related experiments. A combination of these 3 accessions (GSE16471, GSE16472, and GSE17382) exists in GEO. (GSE16557) and has not been imported to PLEXdb because we could not construct a factorial structure from the combination of treatments.

GSE16471: Identification of CPK5ac and CPK11ac early target genes in Arabidopsis mesophyll cells
GSE16472: Identification of early flg22 responsive genes in Arabidopsis mesophyll cells
GSE17382: Identification of early flg22 responsive genes in Arabidopsis seedlings

Publication: 'Differential innate immune signalling via Ca(2+) sensor protein kinases', Boudsocq M, Willmann MR, McCormack M, Lee H, Shan L, He P, Bush J, Cheng SH, Sheen J
Nature 2010 Mar 18;464(7287):418-22
pubmed: 20164835
Created: 2010-08-30 13:44:11
Last Update: 2010-08-31 13:58:40
Released: 2010-09-13
GEO Accession GSE16472
Submitter: PLEXdb Curator
Name: Jen Sheen
Institution: Massachusetts General Hospital, Molecular Biology Department
Head of Laboratory: Jen Sheen
email(s):
Homepage:

 
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