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Arabidopsis thaliana gene expression changes upon treatment with green peach aphid saliva
Georg Jander, Boyce Thompson Institute for Plant Research (gj32@cornell.edu)
Experiment design (6 hybridizations)
insect infestation
•control •M-persicae infested

This experiment has been imported by PLEXdb from NCBI GEO (GSE16497) Series_summary: Myzus persicae (green peach aphid) feeding on Arabidopsis...[complete overview]

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Experiment Name: Arabidopsis thaliana gene expression changes upon treatment with green peach aphid saliva
Accession No: AT83
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
insect infestation
•control   •M-persicae infested
Quality Control: biological replicates
Treatment summary:
 insect infestation  # replicates
 control  3
 M-persicae infested  3
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE16497)

Series_summary:
Myzus persicae (green peach aphid) feeding on Arabidopsis thaliana induces a defense response, quantified as reduced aphid progeny production, in infested leaves but not in other parts of the plant. Similarly, infiltration of aphid saliva into Arabidopsis leaves causes only a local increase in aphid resistance. Further characterization of the defense-eliciting salivary components indicates that Arabidopsis recognizes a proteinaceous elicitor with a size between 3 to 10 kD. Genetic analysis using well-characterized Arabidopsis mutant shows that saliva-induced resistance against M. persicae is independent of the known defense signaling pathways involving salicylic acid, jasmonate, and ethylene. Among 78 Arabidopsis genes that were induced by aphid saliva infiltration, 52 had been identified previously as aphid-induced, but few are responsive to the well-known plant defense signaling molecules salicylic acid and jasmonate. Quantitative PCR analysis confirms expression of saliva-induced genes. In particular, expression of a set of O-methyltransferases, which may be involved in the synthesis of aphid-repellent glucosinolates, was significantly up-regulated by both M. persicae feeding and treatment with aphid saliva. However, this did not correlate with increased production of 4-methoxyindol-3-ylmethylglucosinolate, suggesting that aphid salivary components trigger an Arabidopsis defense response that is independent of this aphid-deterrent glucosinolate.

Series_overall_design:
3 biological replicates (control and treatment). Total number of samples: 6.
Publication: 'Myzus persicae (green peach aphid) salivary components induce defence responses in Arabidopsis thaliana', De Vos M, Jander G
Plant Cell Environ 2009 Nov;32(11):1548-60
pubmed: 19558622
Created: 2010-08-31 14:11:03
Last Update: 2010-08-31 14:24:57
Released: 2010-09-13
GEO Accession GSE16497
Submitter: PLEXdb Curator
Name: Georg Jander
Institution: Boyce Thompson Institute for Plant Research
Head of Laboratory: --
email(s):
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