Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse AraPLEX Experiment Data

Choose an experiment:
Transcript profile of chitosan-treated Arabidopsis seedlings
Pierdomenico Perata, Sant'Anna School of Advanced Studies, Crop Physiology Center (p.perata@sssup.it)
Experiment design (4 hybridizations)
treatment
•mock •chitosan

This experiment has been imported by PLEXdb from NCBI GEO (GSE17193) Series_summary: We treated Arabidopsis seedlings with chitosan and carrie...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Transcript profile of chitosan-treated Arabidopsis seedlings
Accession No: AT84
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
treatment
•mock   •chitosan
Quality Control: biological replicates
Treatment summary:
 treatment  # replicates
 mock  2
 chitosan  2
Total hybridizations: 4
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE17193)

Series_summary:
We treated Arabidopsis seedlings with chitosan and carried out a transcript profiling analysis (GeneChip microarrays) in order to identify genes and transcription factors regulated by chitosan. The results showed that jasmonate and defense responsive genes, camalexin and lignin biosynthetic genes were among genes up-regulated by chitosan. Several transcription factors are also strongly induced by chitosan.
The results suggested that chitosan can be used as a strong elicitor of defense pathways.

Series_overall_design:
Arabidopsis thaliana, ecotype Columbia (Col-0) seeds were sterilized for 7 min in 1.7% (v/v) bleach solution, incubated over night in 4% PPM (Plant Preservative Mixture, Plant Cell Technology, Washington DC, USA) in a full strength sterilized Murashige-Skoog (MS) salt solution with gentle shaking. Subsequently, the seeds were rinsed in abundant sterile water and transferred into 2.5 ml liquid growing media (MS half strength solution) with 0.05% PPM in 6-well plates. The plates were incubated in the dark at 4 °C for two days and finally transferred to continuous light (90µm photons/ m-2) with gentle swirling for four days in a plant growth chamber at 22 °C.
. Treatments were performed by comparing a control solution containing the solvent used to solubilize chitosan, and a chitosan solution concentrated 150 µg/ml.
Publication: none
Created: 2010-08-31 14:30:54
Last Update: 2010-08-31 14:49:25
Released: 2010-09-14
GEO Accession GSE17193
Submitter: PLEXdb Curator
Name: Pierdomenico Perata
Institution: Sant'Anna School of Advanced Studies, Crop Physiology Center
Head of Laboratory: --
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters