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Expression data from Arabidopsis plants misexpressing AtMYB30 after Xanthomonas inoculation at early timepoints
Dominique ROBY, CNRS/INRA, Laboratoire des Interactions Plantes - Microorganismes (dominique.roby@toulouse.inra.fr)
Experiment design (18 hybridizations)
time
•0 hrs •90-105 mins •2-4 hrs •6 hrs
strain
•Wildtype •AtMYB30

This experiment has been imported by PLEXdb from NCBI GEO (GSE9674) Series_summary: Plant immune responses to pathogen attack involve various ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression data from Arabidopsis plants misexpressing AtMYB30 after Xanthomonas inoculation at early timepoints
Accession No: AT87
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
time
•0 hrs  •90-105 mins  •2-4 hrs  •6 hrs
strain
•Wildtype   •AtMYB30
Quality Control: biological replicates
Treatment summary:
 time  strain  # replicates
 0 hrs  Wildtype  2
 0 hrs  AtMYB30  2
 90-105 mins  Wildtype  4
 90-105 mins  AtMYB30  4
 2-4 hrs  Wildtype  2
 2-4 hrs  AtMYB30  2
 6 hrs  Wildtype  1
 6 hrs  AtMYB30  1
Total hybridizations: 18
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE9674)

Series_summary:
Plant immune responses to pathogen attack involve various defense mechanisms and among them, the Hypersensitive Response (HR), a form of programmed cell death occurring at invasion sites. AtMYB30, a transcription factor acts as a positive regulator of a cell death pathway conditioning the HR.
We show by microarray analyses of Arabidopsis plants misexpressing AtMYB30 that the genes encoding the four enzymes forming the acyl-coA elongase complex, responsible for very long chain fatty acids (VLCFA) biosynthesis, are putative targets.
Keywords: Time course after inoculation with a Xanthomonas strain, Xcc147, in Arabidopsis wild-type plants and transgenic plants (AtMYB30 overexpressor (ox) and antisense (as) lines

Series_overall_design:
18 samples are analyzed : Leaves of 4 week-old plants syringe-infiltrated with the Xcc147 strain (or not) were harvested. In experiment 1, we selected 4 timepoints : T0 (not inoculated), 90-105 min, 2-4 h and 6 h post inoculation in the wild-type line, and in a transgenic line overexpressing AtMYB30. Experiment 2 is a biological replicate of experiment 1 (except for 6 hpi). In experiment 3, we focused on the early timepoint 90-105 min to compare the wild-type line and an AtMYB30 antisense line. Experiment 4 is a biological replicate of experiment 3. Plant material was pooled when necessary, and frozen before RNA extraction and hybridization on Affymetrix ATH1 microarrays. As a screen for candidate target genes, we looked for genes, i) up-regulated in the wild-type line after inoculation with Xcc147 as compared to time T0 (not inoculated) , ii) over-expressed in the AtMYB30 overexpressor line compared to the wild-type, and iii) down-regulated in the AtMYB30 antisense line compared to the wild-type.
Publication: 'A MYB transcription factor regulates very-long-chain fatty acid biosynthesis for activation of the hypersensitive cell death response in Arabidopsis', Raffaele S, Vailleau F, Léger A, Joubès J, Miersch O, Huard C, Blée E, Mongrand S, Domergue F, Roby D
Plant Cell. 2008 Mar;20(3):752-67. Epub 2008 Mar 7
pubmed: 18326828
Created: 2010-09-07 12:41:38
Last Update: 2010-09-13 14:09:31
Released: 2010-09-20
GEO Accession GSE9674
Submitter: PLEXdb Curator
Name: Dominique ROBY
Institution: CNRS/INRA, Laboratoire des Interactions Plantes - Microorganismes
Head of Laboratory: --
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