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Indolic metabolites are required for antifungal defense of the Arabidopsis mlo2 mutant
Emiel Ver Loren van Themaat, Max Planck Institute, Department of Plant-Microbe Interactions (themaat@mpiz-koeln.mpg.de,panstrug@mpiz-koeln.mpg.de)
Experiment design (6 hybridizations)
genotype
•Wildtype •mlo-triple mutant
time
•5 weeks •6 weeks •7 weeks

This experiment has been imported by PLEXdb from NCBI GEO (GSE17875) Series_summary: Arabidopsis thaliana genes MLO2 (Mildew resistance locus-...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Indolic metabolites are required for antifungal defense of the Arabidopsis mlo2 mutant
Accession No: AT89
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Wildtype   •mlo-triple mutant
time
•5 weeks  •6 weeks  •7 weeks
Quality Control: biological replicates
Treatment summary:
 genotype  time  # replicates
 Wildtype  5 weeks  1
 Wildtype  6 weeks  1
 Wildtype  7 weeks  1
 mlo-triple mutant  5 weeks  1
 mlo-triple mutant  6 weeks  1
 mlo-triple mutant  7 weeks  1
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE17875)

Series_summary:
Arabidopsis thaliana genes MLO2 (Mildew resistance locus-O 2), MLO6 and MLO12 exhibit unequal genetic redundancy with respect to the modulation of defense responses against powdery mildew fungi and the control of developmental phenotypes such as premature leaf decay. We show that early chlorosis and necrosis of rosette leaves in mlo2 mlo6 mlo12 mutants reflects an authentic but untimely leaf senescence program. Comparative transcriptional profiling revealed that transcripts of several genes encoding tryptophan/indole biosynthetic enzymes hyper-accumulate during vegetative development in the mlo2 mlo6 mlo12 mutant. Elevated expression levels of these genes correlate with altered steady-state levels of several indolic metabolites, including the phytoalexin camalexin and indolic glucosinolates, during development in the mlo2 single and the mlo2 mlo6 mlo12 triple mutant. Results of genetic epistasis analysis suggest a decisive role for indolic metabolites in mlo2-conditioned antifungal defense against both biotrophic powdery mildews and a camalexin-sensitive strain of the necrotrophic fungus, Botrytis cinerea. The wound- and pathogen-responsive callose synthase Powdery mildew resistance 4/Glucan-synthase-like 5 (PMR4/GSL5) was found to be responsible for the spontaneous callose deposits in mlo2 mutant plants but dispensable for mlo2-conditioned penetration resistance. Our data strengthen the notion that powdery mildew resistance of mlo2 genotypes is based on the same defense execution machinery as innate antifungal immune responses that restrict invasion of non-adapted fungal pathogens.

Series_overall_design:
Mature rosette leaves from WT and mlo2 mlo6 mlo12 triple mutant plants were harvested at 5, 6 and 7 weeks after sowing. 6 arrays.
Publication: 'Tryptophan-derived metabolites are required for antifungal defense in the Arabidopsis mlo2 mutant.', Consonni C, Bednarek P, Humphry M, Francocci F, Ferrari S, Harzen A, Ver Loren van Themaat E, Panstruga R.
Plant Physiol. 2010 Mar;152(3):1544-61. Epub 2009 Dec 18.
pubmed: 20023151
Created: 2010-09-14 12:55:53
Last Update: 2010-09-14 13:29:51
Released: 2010-09-22
GEO Accession GSE17875
Submitter: PLEXdb Curator
Name: Emiel Ver Loren van Themaat
Institution: Max Planck Institute, Department of Plant-Microbe Interactions
Head of Laboratory: Ralph Panstruga
email(s):
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