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Browse AraPLEX Experiment Data

Choose an experiment:
Arabidopsis thaliana mutant leaves treated with Pseudomonas syringae ES4326
Raka Mitra, -- (jglazebr@umn.edu)
Experiment design (24 hybridizations)
strain
•coi1 •Col0 •ein2 •npr1 •pad2 •pad4 •sid2
pathogen inoculation
•mock •PsmES4326

This experiment has been imported by PLEXdb from NCBI GEO (GSE18978) Series_summary: In order to better understand the transcriptional network...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Arabidopsis thaliana mutant leaves treated with Pseudomonas syringae ES4326
Accession No: AT91
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
strain
•coi1   •Col0   •ein2   •npr1   •pad2   •pad4   •sid2
pathogen inoculation
•mock   •PsmES4326
Quality Control: biological replicates
Treatment summary:
 strain  pathogen inoculation  # replicates
 coi1  mock  0
 coi1  PsmES4326  3
 Col0  mock  3
 Col0  PsmES4326  3
 ein2  mock  0
 ein2  PsmES4326  3
 npr1  mock  0
 npr1  PsmES4326  3
 pad2  mock  0
 pad2  PsmES4326  3
 pad4  mock  0
 pad4  PsmES4326  3
 sid2  mock  0
 sid2  PsmES4326  3
Total hybridizations: 24
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE18978)

Series_summary:
In order to better understand the transcriptional networks triggered by pathogen inoculation, we monitored gene expression in leaves of mutant Arabidopsis plants, inoculated with Pseudomonas syringae ES4326 and wild type Col-0 plants grown in parallel. Individual leaves were injected in the morning using a needle-less syringe with 10E5 cfu cm-2 PsmES4326 (suspended in 5 mM MgSO4). For the wild type, leaves were also mock treated with 5 mM MgSO4. Leaves were harvested 24 hours later. Plants were grown in pots with BM-2 soil (Berger Peat Moss Ltd, Quebec, Canada) at a density of 9 plants per pot and kept at 22 degrees Celsius with 75% humidity and a 12 hour day length.

Keywords: Expression profilling by array

Series_overall_design:
24 samples were used in this experiment
Publication: none
Created: 2010-09-15 13:51:00
Last Update: 2010-09-15 14:18:27
Released: 2010-10-26
GEO Accession GSE18978
Submitter: PLEXdb Curator
Name: Raka Mitra
Institution: --
Head of Laboratory: Jane Glazebrook
email(s):
Homepage:

 
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