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Systemic responses to Pseudomonas syringae infection
Dr. William Truman, University of Exeter, School of Biosciences (m.r.grant@exeter.ac.uk)
Experiment design (27 hybridizations)
time
•8 hai •12 hai •21 hai
pathogen strain
•DC3000 wildtype •hrpA mutant •avrRpm1

This experiment has been imported by PLEXdb from NCBI GEO (GSE19264) Series_summary: We used three different strains of Pseudomonas syringae p...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Systemic responses to Pseudomonas syringae infection
Accession No: AT94
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
time
•8 hai  •12 hai  •21 hai
pathogen strain
•DC3000 wildtype   •hrpA mutant   •avrRpm1
Quality Control: biological replicates
Treatment summary:
 time  pathogen strain  # replicates
 8 hai  DC3000 wildtype  3
 8 hai  hrpA mutant  3
 8 hai  avrRpm1  3
 12 hai  DC3000 wildtype  3
 12 hai  hrpA mutant  3
 12 hai  avrRpm1  3
 21 hai  DC3000 wildtype  3
 21 hai  hrpA mutant  3
 21 hai  avrRpm1  3
Total hybridizations: 27
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE19264)

Series_summary:
We used three different strains of Pseudomonas syringae pv tomato DC3000 to investigate systemic responses to infection in Arabidopsis and the development of SAR. Wildtype DC3000, the hrpA mutant and DC3000 carrying the avirulence gene avrRpm1 were syringe infiltrated into 4-week-old plants at a concentration of 10e8 cfu/ml. At least 5 leaves per plant were infiltrated and at least 10 plants were pooled for each sample. Systemic, uninfected tissue was then harvested at 8, 12 and 21h after inoculation. Three independent experiments were carried out to give three biological replicates for each timepoint.

Series_overall_design:
27 samples were used in this experiment.
Publication: none
Created: 2010-09-21 12:06:34
Last Update: 2010-09-21 12:42:18
Released: 2010-10-04
GEO Accession GSE19264
Submitter: PLEXdb Curator
Name: Dr. William Truman
Institution: University of Exeter, School of Biosciences
Head of Laboratory: Prof. Murray Grant
email(s):
Homepage:

 
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