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Biological function of Pip3
Dr. Yves Marco, Laboratoire des Inter Plantactionses Micro-Organismes, Unité Mixte de Recherche, Centre National de la Recherche Scientifique–Institut National de la Recherche Agronomique 2594/441 (pascal.gamas@toulouse.inra.fr)
Experiment design (27 hybridizations)
genetic line
•KO-Pip3 •pGli-69-24 •pGli-69-31 •Pip3-3HA6H-5 •Pip3-3HA6H-1 •Pip3-3HA-9 •Pip3-3HA-1 •Nd-1 •Col-0

This experiment has been imported by PLEXdb from NCBI GEO (GSE19273) Series_summary: Ralstonia solanacearum is the causal agent of bacterial w...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Biological function of Pip3
Accession No: AT95
Microarray: ATH1-121501
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•KO-Pip3   •pGli-69-24   •pGli-69-31   •Pip3-3HA6H-5   •Pip3-3HA6H-1   •Pip3-3HA-9   •Pip3-3HA-1   •Nd-1   •Col-0
Quality Control: biological replicates
Treatment summary:
 genetic line  # replicates
 KO-Pip3  3
 pGli-69-24  3
 pGli-69-31  3
 Pip3-3HA6H-5  3
 Pip3-3HA6H-1  3
 Pip3-3HA-9  3
 Pip3-3HA-1  3
 Nd-1  3
 Col-0  3
Total hybridizations: 27
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE19273)

Series_summary:
Ralstonia solanacearum is the causal agent of bacterial wilt. Arabidopsis thaliana monogenic resistance to the strain GMI1000 is conferred by the RRS1-R gene, present in the resistant ecotype ND-1 and absent in the susceptible ecotype Col-0. The corresponding protein is recognized by the avirulence protein PopP2 of R. solanacearum. In order to identify the plant proteins involved in the PopP2/RRS1-R perception complex, the screening of a root cDNA library in yeast was performed using PopP2 as bait. One interactor found was called Pip3, and we would like to better understand its biological function. Thereby we choose to compare two RNAi lines with their wild type Nd-1 background, and one KO line with its wild type Col-0 background. Also, two overexpressor lines will be compared with their wilt type Nd-1 background and two with their Col-0 background.

Series_overall_design:
27 samples were used in this experiment.

PLEXdb Curator's Note:
We are unable to incorporate the RNAi and Overexpressor lines as treatment factors since we can't distinguish them from the limited description available.
Publication: none
Created: 2010-09-21 12:48:24
Last Update: 2010-09-21 13:24:28
Released: 2010-10-05
GEO Accession GSE19273
Submitter: PLEXdb Curator
Name: Dr. Yves Marco
Institution: Laboratoire des Inter Plantactionses Micro-Organismes, Unité Mixte de Recherche, Centre National de la Recherche Scientifique–Institut National de la Recherche Agronomique 2594/441
Head of Laboratory: Dr. Pascal Gamas
email(s):
Homepage:

 
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