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Browse BarleyBase Experiment Data

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Cross-species Detection in Barley1 GeneChip Array
Rico Caldo, Iowa State University (racaldo@iastate.edu)
Experiment design (12 hybridizations)
species
•Barley •Wheat •Oat •Sorghum •Corn •Rice

This study was conducted to evaluate the efficiency of cross-species detection in Barley1 GeneChip array. We hybridized cRNA derived from first le...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Cross-species Detection in Barley1 GeneChip Array
Accession No: BB1
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
species
•Barley  •Wheat  •Oat  •Sorghum  •Corn  •Rice
Quality Control: biological replicates
Treatment summary:
 species  # replicates
 Barley  2
 Wheat  2
 Oat  2
 Sorghum  2
 Corn  2
 Rice  2
Total hybridizations: 12
Description: This study was conducted to evaluate the efficiency of cross-species detection in Barley1 GeneChip array. We hybridized cRNA derived from first leaves of barley green seedlings (as a control), as well as the same stage of seedling leaf from representative genotypes of wheat, oat, rice, maize, and sorghum. Ten to twenty seedlings for each species were harvested and pooled for RNA preparation, labeling, and hybridization.

Submitted at NCBI GEO via PLEXdb.

Publication: 'A New Resource for Cereal Genomics: 22K Barley GeneChip Comes of Age', Close TJ, Wanamaker SI, Caldo RA, Turner SM, Ashlock DA, Dickerson JA, Wing RA, Muehlbauer GJ, Kleinhofs A, Wise RP
Plant Physiol. 2004 Mar;134(3):960-8
pubmed: 15020760
Created: 2004-06-10 09:18:42
Last Update: 2011-10-11 13:00:25
GEO Accession GSE33391
Submitter: Rico A. Caldo
Name: Rico Caldo
Institution: Iowa State University
Head of Laboratory: Roger P Wise
email(s):
Homepage:

 
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