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Transcript profiling of Bln1 silenced plants (BSMV-VIGS) relative to empty vector and buffer treated controls in barley-powdery mildew interactions
Yan Meng, Iowa State University (yanmeng@iastate.edu)
Experiment design (60 hybridizations)
genotype
•Clansman (Mla13) •HOR11358 (Mla9)
treatedoruntreated
•Inoc. w/ Bgh 5874 •Non-Inoc.
treatment
•Mock (Buffer Control) •BSMV:00 •BSMV:Bln1[248]

Barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) was used to identify significant new genes in the regulation of host innate immunity....[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcript profiling of Bln1 silenced plants (BSMV-VIGS) relative to empty vector and buffer treated controls in barley-powdery mildew interactions
Accession No: BB101
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Clansman (Mla13)   •HOR11358 (Mla9)
treatedoruntreated
•Inoc. w/ Bgh 5874   •Non-Inoc.
treatment
•Mock (Buffer Control)   •BSMV:00   •BSMV:Bln1[248]
Quality Control: biological replicates
Treatment summary:
 genotype  treatedoruntreated  treatment  # replicates
 Clansman (Mla13)  Inoc. w/ Bgh 5874  Mock (Buffer Control)  5
 Clansman (Mla13)  Inoc. w/ Bgh 5874  BSMV:00  5
 Clansman (Mla13)  Inoc. w/ Bgh 5874  BSMV:Bln1[248]  5
 Clansman (Mla13)  Non-Inoc.  Mock (Buffer Control)  5
 Clansman (Mla13)  Non-Inoc.  BSMV:00  5
 Clansman (Mla13)  Non-Inoc.  BSMV:Bln1[248]  5
 HOR11358 (Mla9)  Inoc. w/ Bgh 5874  Mock (Buffer Control)  5
 HOR11358 (Mla9)  Inoc. w/ Bgh 5874  BSMV:00  5
 HOR11358 (Mla9)  Inoc. w/ Bgh 5874  BSMV:Bln1[248]  5
 HOR11358 (Mla9)  Non-Inoc.  Mock (Buffer Control)  5
 HOR11358 (Mla9)  Non-Inoc.  BSMV:00  5
 HOR11358 (Mla9)  Non-Inoc.  BSMV:Bln1[248]  5
Total hybridizations: 60
Description: Barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) was used to identify significant new genes in the regulation of host innate immunity. This experiment was designed to uncover significant changes in Bln1 (Contig12219_at)-silenced plants relative to empty vector and buffer treated controls. Five independent biological replications of a split-plot experimental design were conducted with replications as blocks, treatment with Blumeria graminis f. sp. hordei (Bgh) as the whole-plot factor, and all combinations of genotype (Mla13 and Mla9) and VIGS treatment [Buffer control (mock), BSMV:00 (empty vector), and BSMV:Bln1248] as the split-plot factor for a total of 60 GeneChip hybridizations. Ten seedlings were used as a split-plot experimental unit for each combination of replication, Bgh treatment, genotype, and VIGS treatment. Plants were grown in a controlled 20°C glasshouse prior to VIGS treatment. Twelve days after VIGS treatment, half of the plants in each replication were challenged with the compatible Bgh isolate 5874. Top halves of 5 of the 10 seedling third leaves (about 10 cm) from each split-plot experimental unit were harvested into liquid N2 at 32 hours after inoculation (HAI) - the timepoint with the highest differential Bln1 transcript accumulation (Meng et al. 2009), and after initial establishment of the perihaustorial interface (Caldo et al. 2004). The remaining 5 leaves were used to record infection phenotype 7 days later. RNA was isolated for GeneChip hybridization from the 32-HAI samples.

Submitted at NCBI GEO via PLEXdb.

Publication: 'Blufensin1 negatively impacts basal defense in response to barley powdery mildew', Meng, Y, MJ Moscou, and RP Wise
Plant Physiology 149: 271-285
pubmed: 19005086
Created: 2009-11-05 15:19:59
Last Update: 2011-02-15 14:54:49
Released: 2014-09-30
GEO Accession GSE61644
Submitter: Greg S Fuerst
Name: Yan Meng
Institution: Iowa State University
Head of Laboratory: Roger P Wise, USDA-ARS
email(s):
Homepage:

 
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