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Experiment Name:

Transcript profiling of Bln1 silenced plants (BSMV-VIGS) relative to empty vector and buffer treated controls in barley-powdery mildew interactions

Accession No:

BB101

Microarray:

Barley1

Visibility:

public

Experiment Type:

Experiment Factor(s):

genotype: •Clansman (Mla13) •HOR11358 (Mla9)
treatedoruntreated: •Inoc. w/ Bgh 5874 •Non-Inoc.
treatment: •Mock (Buffer Control) •BSMV:00 •BSMV:Bln1[248]

Quality Control:

biological replicates

Treatment summary:

genotype	treatedoruntreated	treatment	# replicates
Clansman (Mla13)	Inoc. w/ Bgh 5874	Mock (Buffer Control)	5
Clansman (Mla13)	Inoc. w/ Bgh 5874	BSMV:00	5
Clansman (Mla13)	Inoc. w/ Bgh 5874	BSMV:Bln1[248]	5
Clansman (Mla13)	Non-Inoc.	Mock (Buffer Control)	5
Clansman (Mla13)	Non-Inoc.	BSMV:00	5
Clansman (Mla13)	Non-Inoc.	BSMV:Bln1[248]	5
HOR11358 (Mla9)	Inoc. w/ Bgh 5874	Mock (Buffer Control)	5
HOR11358 (Mla9)	Inoc. w/ Bgh 5874	BSMV:00	5
HOR11358 (Mla9)	Inoc. w/ Bgh 5874	BSMV:Bln1[248]	5
HOR11358 (Mla9)	Non-Inoc.	Mock (Buffer Control)	5
HOR11358 (Mla9)	Non-Inoc.	BSMV:00	5
HOR11358 (Mla9)	Non-Inoc.	BSMV:Bln1[248]	5

Total hybridizations: 60

Description:

Barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) was used to identify significant new genes in the regulation of host innate immunity. This experiment was designed to uncover significant changes in Bln1 (Contig12219_at)-silenced plants relative to empty vector and buffer treated controls. Five independent biological replications of a split-plot experimental design were conducted with replications as blocks, treatment with Blumeria graminis f. sp. hordei (Bgh) as the whole-plot factor, and all combinations of genotype (Mla13 and Mla9) and VIGS treatment [Buffer control (mock), BSMV:00 (empty vector), and BSMV:Bln1248] as the split-plot factor for a total of 60 GeneChip hybridizations. Ten seedlings were used as a split-plot experimental unit for each combination of replication, Bgh treatment, genotype, and VIGS treatment. Plants were grown in a controlled 20°C glasshouse prior to VIGS treatment. Twelve days after VIGS treatment, half of the plants in each replication were challenged with the compatible Bgh isolate 5874. Top halves of 5 of the 10 seedling third leaves (about 10 cm) from each split-plot experimental unit were harvested into liquid N2 at 32 hours after inoculation (HAI) - the timepoint with the highest differential Bln1 transcript accumulation (Meng et al. 2009), and after initial establishment of the perihaustorial interface (Caldo et al. 2004). The remaining 5 leaves were used to record infection phenotype 7 days later. RNA was isolated for GeneChip hybridization from the 32-HAI samples.

Submitted at NCBI GEO via PLEXdb.

Publication:

'Blufensin1 negatively impacts basal defense in response to barley powdery mildew', Meng, Y, MJ Moscou, and RP Wise
Plant Physiology 149: 271-285
pubmed: 19005086

Created:

2009-11-05 15:19:59

Last Update:

2011-02-15 14:54:49

Released:

2014-09-30

GEO Accession

GSE61644

Submitter:

Greg S Fuerst

Experimenter:

Name:	Yan Meng
Institution:	Iowa State University
Head of Laboratory:	Roger P Wise, USDA-ARS
email(s):	yanmeng@iastate.edu
Homepage:	www.wiselab.org


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