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Heat stress expression data from developing barley caryopses
Joachim Kilian, University of Tuebingen, Department of ZMBP Plant Physiology, Tuebingen, Germany (joachim.kilian@zmbp.uni-tuebingen.de,elke.mangelsen@vbsg.slu.se)
Experiment design (12 hybridizations)
stress condition
•control •heat stress
time
•0.5 hrs •3 hrs •6 hrs

This experiment has been imported by PLEXdb from NCBI GEO (GSE23896) Series_summary: High temperature stress, like any abiotic stress, impairs...[complete overview]

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Experiment Name: Heat stress expression data from developing barley caryopses
Accession No: BB102
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
stress condition
•control   •heat stress
time
•0.5 hrs  •3 hrs  •6 hrs
Quality Control: biological replicates
Treatment summary:
 stress condition  time  # replicates
 control  0.5 hrs  2
 control  3 hrs  2
 control  6 hrs  2
 heat stress  0.5 hrs  2
 heat stress  3 hrs  2
 heat stress  6 hrs  2
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE23896)

Series_summary:
High temperature stress, like any abiotic stress, impairs the physiology and development of plants, including the stages of seed setting and ripening.
In this study we used the 22K Barley1 GeneChip microarray to investigate the response of developing barley (Hordeum vulgare) caryopses at 12 days post anthesis to 0.5h, 3h and 6h of heat stress exposure.

Series_overall_design:
Developing barley caryopses were harvested at different time-points of a day during heat stress (42°C) (2:00 PM, 5:00 PM and 8:00 PM). Heat stress started at 1:30PM. Pooled caryopses (form 3 plants) from the respective time points were used for RNA extraction and hybridization on Affymetrix microarrays (Barley1 GeneChip; GEO accsession GPL1340). Biological replicates were performed.

PLEXdb Curator's Note:
The time points have been adopted from the experiment summary and the publication rather than from the sample titles in GEO.
Publication: 'Transcriptome analysis of high-temperature stress in developing barley caryopses: early stress responses and effects on storage compound biosynthesis.', Mangelsen E, Kilian J, Harter K, Jansson C, Wanke D, Sundberg E.
Mol Plant 2011 Jan;4(1):97-115.
pubmed: 20924027
Created: 2011-03-31 12:49:18
Last Update: 2011-03-31 13:09:12
Released: 2011-04-05
GEO Accession GSE23896
Submitter: PLEXdb Curator
Name: Joachim Kilian
Institution: University of Tuebingen, Department of ZMBP Plant Physiology, Tuebingen, Germany
Head of Laboratory: Elke Mangelsen
email(s):
Homepage:

 
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