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Browse BarleyBase Experiment Data

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Microarray data from barley aleurone
Kegui Chen, University of Wisconsin, Department of Agronomy, Madison, Wisconsin (kchen@desu.edu)
Experiment design (15 hybridizations)
compound
•untreated •GA •ABA •GA plus ABA
genotype
•control •sln1 mutant

This experiment has been imported by PLEXdb from NCBI GEO (GSE18758)

Series_summary:
The phytohormone gibberellic acid (GA) is well k...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Microarray data from barley aleurone
Accession No: BB103
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
compound
•untreated   •GA   •ABA   •GA plus ABA
genotype
•control   •sln1 mutant
Quality Control: biological replicates
Treatment summary:
 compound  genotype  # replicates
 untreated  control  3
 untreated  sln1 mutant  3
 GA  control  3
 GA  sln1 mutant  0
 ABA  control  3
 ABA  sln1 mutant  0
 GA plus ABA  control  3
 GA plus ABA  sln1 mutant  0
Total hybridizations: 15
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE18758)

Series_summary:
The phytohormone gibberellic acid (GA) is well known to promote seed germination in plants. One of its functions is to stimulate the production of hydrolytic enzymes in the aleurone and their secretion to the adjacent endosperm. The storage in the endosperm is thus degraded by these hydrolases into small molecules, which are utilized as nutrients for embryo growth to establish the young seedling. ABA in contrast plays antagonistic role to GA to keep seed in dormancy.
Cereal aleurone has been established as a model system to investigate giberrellin (GA) and abscisic acid (ABA) responses. Using Barley 1 GeneChip, we examined the mRNA accumulation of over 22 000 genes in barley aleurone treated with GA, ABA, GA plus ABA, and sln1 mutant.

Series_overall_design:
Barley aleurone tissues were separated from half-seed without embryo. Three independent RNA samples for each treatment including the control without any hormone were extracted and hybridized onto Affymetrix microarrays. We also did microarray in three replications for sln1 mutant aleurone without hormone treatment.
Publication: 'Transcriptional Responses to Gibberellin and Abscisic Acid in Barley Aleurone.', Kegui Chen and Yong-Qiang Charles An.
Journal of Integrative Plant Biology, Volume 48 Issue 5, Pages 591 - 612.'Loss-of-function of DELLA protein SLN1 activates GA signaling in barley aleurone', Kegui Chen, Shulan Tian, Brian S. Yandell, Shawn M. Kaeppler and Yong-qiang Charles An
Acta Physiologiae Plantarum. doi:10.1007/s11738-010-0464-3
Created: 2011-05-17 10:07:01
Last Update: 2011-06-20 15:17:17
Released: 2011-06-20
GEO Accession GSE18758
Submitter: PLEXdb Curator
Name: Kegui Chen
Institution: University of Wisconsin, Department of Agronomy, Madison, Wisconsin
Head of Laboratory: Yong-Qiang Charles An
email(s):
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