Search   for 
Find Your Gene ·  Publications ·  Tools ·  Gene List Suite ·  Expression Atlases ·  About PLEXdb ·  Feedback
Login ID:
Password:
Plants
Arabidopsis
Barley
Brachypodium
Citrus
Cotton
Grape
Maize
Medicago
Poplar
Rice
Soybean
Sugarcane
Tomato
Wheat
Pathogens & Symbionts
Search Experiments
Download Data
Gene List Suite
Tools
Your Account
Documentation
News
Personnel
Links
 Data Release Policy 
PLEXdb is committed to making expression data publicly available to researchers world-wide...
complete policy
 

Browse BarleyBase Experiment Data

Choose an experiment:
Diurnal expression data from developing barley caryopses
Joachim Kilian, University of Tuebingen, Department of ZMBP Plant Physiology, Tuebingen, Germany (joachim.kilian@zmbp.uni-tuebingen.de)
Experiment design (12 hybridizations)
time
•1h(dark) •9h(dark) •10h(light) •17h(light) •20h(light) •23h(light)

This experiment has been imported by PLEXdb from NCBI GEO (GSE20034)

Series_summary:
Caryopses of barley (Hordeum vulgare), like all ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

Show
Experiment Name: Diurnal expression data from developing barley caryopses
Accession No: BB104
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
time
•1h(dark)   •9h(dark)   •10h(light)   •17h(light)   •20h(light)   •23h(light)
Quality Control: biological replicates
Treatment summary:
 time  # replicates
 1h(dark)  2
 9h(dark)  2
 10h(light)  2
 17h(light)  2
 20h(light)  2
 23h(light)  2
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE20034)

Series_summary:
Caryopses of barley (Hordeum vulgare), like all other cereal seeds, are complex sink organs optimized for storage starch accumulation and embryo development. Their development from early stages after pollination to late stages of seed ripening has been studied in great detail. However, information on the caryopses’ diurnal adaptation to changes in light, temperature and alterations in phloem-supplied carbon and nitrogen remained unknown.
In this study, we applied the 22K Barley1 GeneChip microarray to investigate diurnal gene regulation events of barley caryopses at 11 to 12 days post anthesis.

Series_overall_design:
Developing barley caryopses were harvested at different time-points of a day (at 7:00 AM, 2:00 PM, 5:00 PM and 8:00 PM) including dark samples (6:00 AM and 10:00 PM). Pooled caryopses (form 3 Plants) from the respective time points were used for RNA extraction and hybridization on Affymetrix microarrays (Barley1 GeneChip; GEO accsession GPL1340). Biological replicates were performed.
Publication: 'Significance of light, sugar, and amino acid supply for diurnal gene regulation in developing barley caryopses.', Mangelsen E, Wanke D, Kilian J, Sundberg E, Harter K, Jansson C.
Plant Physiol 2010 May;153(1):14-33.
pubmed: 20304969
Created: 2011-05-17 11:01:59
Last Update: 2011-06-21 10:28:13
Released: 2011-06-21
GEO Accession GSE20034
Submitter: PLEXdb Curator
Name: Joachim Kilian
Institution: University of Tuebingen, Department of ZMBP Plant Physiology, Tuebingen, Germany
Head of Laboratory: Elke Mangelsen
email(s):
Homepage:

 
Copyright@2001-2013 The PLEXdb Group
All rights reserved.

For problems with the webpages contact PLEXdb webmasters