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Browse BarleyBase Experiment Data

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Transcriptome analysis of the barley fast neutron mutants nec3
Nils Rostoks, University of Latvia, Riga, Latvia (nils.rostoks@lu.lv)
Experiment design (6 hybridizations)
genotype
•FN362 •FN363 •Steptoe(wt)

This experiment has been imported by PLEXdb from NCBI GEO (GSE23775)

Series_summary:
Affymetrix Barley1 GeneChip microarray was used ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcriptome analysis of the barley fast neutron mutants nec3
Accession No: BB105
Microarray: Barley1
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•FN362   •FN363   •Steptoe(wt)
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 FN362  2
 FN363  2
 Steptoe(wt)  2
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE23775)

Series_summary:
Affymetrix Barley1 GeneChip microarray was used to study the transcriptome of two fast neutron barley mutants FN362 and FN363 allelic to characterized necrotic leaf spot 3 (nec3) mutants. PCR screening of the 21 genes at least two-fold down-regulated in mutants did not show any deletions in the genome of mutants and, thus, failed to identify a gene responsible for the nec3 mutant phenotype suggesting that either probe set for the NEC3 gene is not on the Barley1 GeneChip, or that the expression of wt NEC3 is confined to specific developmental stage or tissue type.

Series_overall_design:
Transcriptome of the two barley fast neutron mutants was compared to the transcriptome of parental variety Steptoe
Publication: 'Transcriptome analysis of the barley nec3 mutant reveals a potential link with abiotic stress response related signaling pathways.', Keisa A, Brueggeman R, Drader T, Kleinhofs A, Rostoks N.
Environmental Experimental Biol 2010; 8:1-16.
Created: 2011-05-19 09:06:33
Last Update: 2011-06-21 10:22:48
Released: 2011-06-21
GEO Accession GSE23775
Submitter: PLEXdb Curator
Name: Nils Rostoks
Institution: University of Latvia, Riga, Latvia
Head of Laboratory: Nils Rostoks
email(s):
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